虎刺醛对人肝癌细胞HepG2生长抑制、细胞迁移抑制及其机制的….pdfVIP

中国细胞生物学学报 Chinese Journal of Cell Biology 2013, 35(4): 442–449 虎刺醛对人肝癌细胞HepG2生长抑制、细胞迁移 抑制及其机制的研究 * 丁 兰 柳志军 令利军 贾凌云 第五佳丽 彭 舒 刘国安 (西北师范大学生命科学学院, 兰州 730070) 摘要 采用台盼蓝排染法、划痕法、特异性荧光染色技术检测了虎刺醛对人肝癌细胞 HepG2的生长、细胞迁移、微丝骨架和角蛋白纤维网络的影响以及引起的凋亡作用。结果表明, 15~60 μmol/L 的虎刺醛对HepG2 细胞有明显的生长抑制作用, 并存在浓度和时间依赖性; 低浓度 (15 μmol/L) 的虎刺醛能够显著抑制HepG2细胞迁移, 30 μmol/L几乎完全抑制细胞迁移; 25, 27.5, 30, 35 μmol/L虎刺醛作用HepG2细胞24 h后, 其细胞内的应力纤维束、片状伪足、丝状伪足逐渐减少 或消失, 这与8 μmol/L 细胞松弛素B的处理结果相似; 而25 μmol/L和30 μmol/L虎刺醛对HepG2细胞 角蛋白纤维仅有轻微影响; 15~60 μmol/L 的虎刺醛能不同程度诱导HepG2细胞发生凋亡。虎刺醛 对HepG2细胞内应力纤维(或微丝)的破坏作用极可能是导致细胞生长抑制、迁移抑制的直接原因, 也是使细胞贴壁性降低或丧失从而导致凋亡发生的直接原因。 关键词 虎刺醛; HepG2; 细胞迁移; 应力纤维; 角蛋白纤维; 细胞凋亡 Study of Damnacanthal on Anti-proliferation, Cell Migration Inhibition and Its Mechanism in Human Hepatoma Cell HepG2 Ding Lan*, Liu Zhijun, Ling Lijun, Jia Lingyun, Diwu Jiali, Peng Shu, Liu Guoan (College of Life Science, Northwest Normal University, Lanzhou 730070, China) Abstract The trypan blue staining, wound healing assay, specific fluorescence staining are used to investi- gate the effect of proliferation, cell migration, microfilaments cytoskeleton, keratin filaments network and apoptosis induced by damnacanthal on human hepatoma cells HepG2 respectively. The results demonstrated that damnacan- tha1 significantly inhibited cell proliferation of human hepatoma cell HepG2 at concentrations of 15~60 μmol/L in time and dose dependent manners; 15 μmol/L damnacanthal inhibits cell migration and 30 μmol/L almost inhibits completely; after HepG2 cells were exposed to damnacanthal (25, 27.5, 30, 35 μmol/L) for 24 h, stress fibers, lamel- lipodia and filopodia were absent or decreased gradually with increased concen