干旱胁迫和复水对梨幼树生理特性影响.pdfVIP

2012-04-25 DOI: 10.3724/SP.J.1145.2012.00218 / Chin J Appl Environ Biol 2012 18 ( 2 ) : 218~223 应用与环境生物学报 ， 干旱胁迫和复水对梨幼树 生理特性的影响* 孙继亮 李六林 陶书田 张绍铃** （南京农业大学梨工程技术研究中心 南京 210095） 摘 要 为了解植物干旱生理适应机制和恢复机制，以梨品种‘翠冠’和‘黄冠’幼树为试材，研究土壤干旱胁迫及复 水条件下叶片光合参数、叶片相对含水量、叶片质膜透性、超氧化物歧化酶活性及脯氨酸含量的变化特性 . 结果显示： 1）随着干旱胁迫的加剧，两品种的叶片净光合速率、气孔导度和蒸腾速率均呈下降趋势；‘翠冠’叶片胞间CO 浓度呈 2 先降后升的趋势，而‘黄冠’呈持续下降趋势，但‘黄冠’光合作用的4项指标明显高于‘翠冠’；复水后，两品种的光合 指标恢复显著，但均未超过对照水平. 2）干旱胁迫引起两品种的叶片相对含水量急剧下降，叶片质膜透性和脯氨酸含 量显著升高，超氧化物歧化酶活性呈先升后降趋势；复水后，两品种的4项指标不同程度地得以恢复，但大部分指标 没有恢复到对照水平，其中‘黄冠’各项指标在复水前后的变化幅度比‘翠冠’更小. 综合分析表明，‘翠冠’和‘黄冠’ 对干旱胁迫的响应存在差异，‘黄冠’对干旱逆境具有更强的适应能力. 图2 表 1 参27 关键词 梨树；干旱胁迫；复水；光合参数；相对含水量；质膜透性；超氧化物歧化酶；脯氨酸 CLC Q945.78 : SS����1.2071.207 Effects of Drought Stress and Rewatering on Physiological Characteristics of Pear Seedling* SUN Jiliang, LI Liulin, TAO Shutian ZHANG Shaoling** (Pear Engineering Research Centre, Nanjing Agricultural University, Nanjing 210095, China) Abstract In order to understand the physiological adaptation mechanism and recovery mechanism of plant to drought, the physiological characteristics, including photosynthetic parameters, leaf relative water content, leaf membrane permeability, SOD activity and proline content of pear seedling under drought stress and rewatering conditions were investigated with the varieties ‘Cuiguan’ and ‘Huangguan’ as materials. The results showed that: 1) In the process of drought stress, leaf net photosynthetic rate, stomatal conductance and transpiration rate of the two varieties were decreased. Intercellular CO2 concentration w