基于宏基因组学技术分析污水处理系统中耐药基因和可移动遗传元件分布与丰度.pdfVIP

中国科技论文在线 基于宏基因组学技术分析污水处理系统中 耐药基因和可移动遗传元件分布与丰度# 赵福正，张徐祥** 5 （南京大学环境学院，污染控制与资源化国家重点实验室，南京市 210023 ） 摘要：本文应用PCR、实时定量PCR、Illumina 高通量测序等技术调查了城市污水处理系统 中耐药基因（ARGs）和可移动遗传元件（MGEs）的分布特征。PCR 检测结果表明 15 种四环 素耐药基因（tet）存在于污水处理系统的进水、出水和活性污泥中。运用实时定量PCR 测 10 定8 种tet 基因的丰度，发现进水中tet 丰度最高，其次为出水和活性污泥。Illumina 高 通量测序与生物信息学分析结果表明：污水处理系统中存在多种 ARGs，以及质粒、转座子 和整合子等可移动元件。ARGs 和MGEs 的丰度和多样性在进水中最高，其次为出水和活性污 泥。本研究表明活性污泥工艺可降低多种ARGs 和MGEs 丰度。 关键词：耐药基因；可移动遗传元件；污水处理厂；高通量测序；宏基因组分析 15 中图分类号：X172 A metagenomics analysis on antibiotic resistance genes and mobile genetic elements in a sewage treatment plant ZHAO Fuzheng, ZHANG Xuxiang 20 (State Key Lab of Pollution Control and Resource Reuse, School of the Environment,Nanjing University, Nanjing 210023) Abstract: PCR, quantitative real time PCR and Illumina high-throughput sequencing were used to comprehensively investigate abundance and distribution of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) in a municipal wastewater treatment plant (WWTP). PCRs 25 showed that 15 tetracycline resistance genes (tet) occurred in the sewage, activated sludge and treated sewage. Quantification of 8 tet genes demonstrated that abundance of the tet genes was significantly different among the influent, effluent and sludge samples. The influent water had the highest abundance of tet genes, followed by effluent water and activated sludge. Illumina high-throughput sequencing combined with metagenomic bioinformatics analysis revealed that 30 both diversity and abundance of the detectable ARGs and MG