高效液相色谱测定小儿肺热咳喘口服液中绿原酸与木犀草苷的含量.pdfVIP

422 Medical Science Journal of Central South China,July2013,Vol.41,No.4 文章编号:2095-1116(2013)04-0422-03 ·技术与方法 · 高效液相色谱测定小儿肺热咳喘口服液中 绿原酸与木犀草苷的含量 1 2 2 2 2 李云贵 ,李摇 冲 ,文翔昊 ,鲁启明 ,郭摇 露 (1.长沙医学院药学系,湖南长沙410219;2.南华大学附属南华医院小儿科) 摘摇 要:摇 目的摇 高效液相色谱法(HPLC)测定小儿肺热咳喘口服液中绿原酸与木犀草苷含量。 摇 方法摇 采 用ODS 色谱柱,室温下,以乙腈(A)-0.1%磷酸(B)为流动相直线梯度洗脱:0 min(A:10%;B:90%),30 min(A: 30%;B:70%),流速0.9 mL/ min,检测波长为327 nm。 摇 结果摇 绿原酸和木犀草苷浓度分别在4.0 ~40.0 滋g/ mL 和0.46~4.6 滋g/ mL范围内与峰面积呈良好的线性关系;平均回收率分别为98.6%和98.2%。 摇 结论摇 本方法简 便可行,结果准确可靠,可用于小儿肺热咳口服液的质量控制。 关键词:摇 高效液相色谱法;摇 绿原酸;摇 木犀草苷;摇 小儿肺热咳喘口服液 中图分类号:R284摇 摇 摇 文献标识码:A Determination of Chlorogenic Acid and Galuteolin in Xiao爷er Feire Kechuan Oral Liquid by High Performance Liquid Chromatography LI Yungui,LI Chong,WEN Xianghao,et al (Department of pharmacy,Medical College of Changsha,Hunan,Changsha410219,China) Abstract:摇 Objective摇 To determination of chlorogenic acid and galuteolin in Xiao爷er Feire Kechuan oral liquid by high performance liquid chromatography(HPLC).摇 Methods 摇 Chromatogaphic analysis was performed on ODS column with the room temperature. The mobile phasewas acetonitrile(A) and0.1% phosphoric acid(B) and the linear gradient modewas arranged asfollows:0 min(A:10%;B:90%),30 min(A:30%;B:70%) .The flow rate was0.9 mL ·min-1 and the detection wavelength was327 nm.摇 Results摇 The calibration curvesof chlorogenic acid andgaluteolinwere sepa鄄 rately linear intherangesof4.0~40.0 滋g/ mLand0.46~4.6 滋g/ mLwithcorrelationcoefficient0.9990.Theaveragere鄄 coveries were98.6% and98.2%.摇 Conclusion摇 Themethod is simple,rapid andreliable. So it canbeused asquanti鄄 tative determination