铁矿区内重金属对土壤氨氧化微生物群落组成的影响.pdfVIP

中国环境科学 2014,34(5) ：1212~1221 China Environmental Science 铁矿区内重金属对土壤氨氧化微生物群落组成的影响 洪 晨 1,邢 奕 1,2*,司艳晓 1,2,李 洋 1 (1.北京科技大学土木与环境工程学院,北京 100083 ；2.北京科技大学 金属矿山高效开采与安全教育部重点实验室,北京 100083) 摘要：以密云水库上游某铁矿区为研究对象,采用荧光定量 PCR 和变性梯度凝胶电泳(DGGE)分析了矿区内不同采样点的土壤中氨氧化微 7 9 生物的数量和群落结构的变化,结果表明,土样中氨氧化细菌(AOB)和氨氧化古菌(AOA)的数量变化范围分别为 3.01×10 ~1.08×10 copies/g 干土和 8.65×107 9 ~2.69×10 copies/g 干土.重金属含量与氨氧化微生物数量的相关性分析以及氨氧化微生物群落结构的冗余分析结果表明, 该矿区内重金属污染改变了土壤中的氨氧化微生物的数量和结构.Cu 污染对AOA 的数量起到了显著抑制作用(r= -0.653*, P0.05),但是对 AOB 则没有明显作用;Zn 污染对尾矿库区域土壤的 AOA/AOB 比值影响显著(r= -0.606*, P0.05);Cd 污染改变了 AOB 的种群分布,降低了 AOB 的多样性水平.土壤中 Cr 长期干扰并没有改变氨氧化微生物的数量和结构,但是明显得抑制了氨氧化速率,表明重金属污染在一定程 度上也影响了土壤生态系统的氮循环. 关键词：铁矿区；氨氧化古菌(AOA) ；氨氧化细菌(AOB) ；DGGE ；real-time PCR 中图分类号：X53 文献标识码：A 文章编号：1000-6923(2014)05-1212-10 Impact of long-term heavy metals pollution on ammonia oxidizing microbial community in an iron mine soil. HONG Chen1, XING Yi1,2*, SI Yan-xiao1,2, LI Yang1 (1.Civil and Environmental Engineering School, University of Science and Technology Beijing, Beijing 100083, China；2.Key Laboratory of High Efficiency Mining and Safety for Metal Mine, Ministry of Education, University of Science and Technology Beijing, Beijing 100083, China). China Environmental Science, 2014,34(5)：1212~1221 Abstract ：To examine the changes of ammonia-oxidizing microorganisms community in an iron mine of the upstream area of Miyun Reservoir, soil samples were collected from fourteen sites. Quantification of ammonia-oxidizing bacteria (AOB) and archaea (AOA) were performed by real-time PCR, and the microbial community structures were studied by denaturing gradient gel electrophoresis (DGGE). The results showed that the copy