急性髓细胞白血病细胞的吲哚胺2，3-双加氧酶介导免疫逃逸的实验研究.DOCVIP

临床医学论文-急性髓细胞白血病细胞的吲哚胺2，3-双加氧酶介导免疫逃逸的实验研究 ?????????????? 作者：唐晓琼，赵智刚，王红祥，李秋柏，吕建，邹萍 【摘要】? 为了探讨急性髓细胞白血病细胞的吲哚胺2，3-双加氧酶（IDO）参与肿瘤免疫逃逸的机制，从23例急性髓性白血病患者获取骨髓细胞，采用免疫组织化学染色法和RT-PCR法检测白血病细胞IDO的表达；在有或无1-甲基色氨酸（1-MT）存在下，以白血病细胞为刺激细胞，外周T淋巴细胞为反应细胞建立单向混合淋巴细胞反应体系（MLR），利用MTT法检测T淋巴细胞增殖率，并以反相高效液相色谱法检测相应MLR体系上清液中的IDO活性。结果显示，在23例急性髓细胞白血病患者中17例患者的骨髓白血病细胞上有IDO的表达；白血病细胞上的IDO活性抑制MLR体系中T淋巴细胞的增殖。结论：白血病细胞表达的IDO活性能阻抑外周T淋巴细胞的增殖，它可能参与了肿瘤免疫逃逸。 【关键词】? 急性髓细胞性白血病 　　Indoleamine 2，3-Dioxygenase Activity in Acute Myeloid Leukemia Cells Contributing to Tumor Immune Escape 　　Abstract??? This study was aimed to investigate the mechanism of indoleamine 2，3-dioxygenase (IDO) activity in acute myeloid leukemia cells contributing to tumor immune escape. Myeloid leukemia cells were isolated from bone marrow of 23 patients with acute myeloid leukemia (AML) and IDO expression was detected by immunochemistry and RT-PCR methods.? Then mixed lymphocyte reaction? (MLR) of one way was carried out，leukemia cells? were used as stimulating cells and T-lymphocytes were used as reactive cells in culture with or without 1-MT. T-lymphocyte proliferation rate was determined by MTT assay and IDO activity in supernatant of MLR was detected by high-performance liquid chromatography (HPLC). The results showed that IDO expression was?found in牋 17?out of 23 cases of acute myeloid leukemia cells; IDO enzyme activity in leukemia cells inhibited T-lymphocyte proliferation in?MLR cultures. It is concluded牋 that IDO activity expressing in leukemia cells can suppress T-lymphocyte proliferation responses，which? may be contributing to tumor immune escape. 　　Key words? acute myeloid leukemia; indoleamine 2，3-dioxygenase;? T-lymphocyte;? 1-methyltryptophan; immune escape 　　肿瘤免疫逃逸指肿瘤细胞通过某种机制躲避免疫系统对其监视与杀伤，从而导致肿瘤的发生和复发的现象，迄今为止参与肿瘤的免疫逃逸的具体分子机制尚不清楚［1］。近年的研究发现，小鼠胎盘滋养层细胞吲哚胺2，3-双加氧酶(indoleamine 2，3-dioxygenase，IDO)是防止母鼠异基因Ｔ细胞对胎鼠的免疫排斥反应中的必要因素［2］。同时还发现，抗原呈递细胞（APC）如巨噬细胞、DC上IDO能通过抑止T淋巴细胞的增殖而在免疫系统的反应调控中发挥着重要的作用［3］。另有学者发现，人宫颈癌细胞系HeLa细胞与外周血淋巴细胞共培养后，外周血淋巴细