姜黄素对MPP+诱导PC12细胞凋亡的抑制作用.DOCVIP

临床医学论文-姜黄素对MPP+诱导PC12细胞凋亡的抑制作用 ?????????????? 作者：陈静，李波，李红军，肖长义 【摘要】? 目的 观察姜黄素(Curcumin，Cur)对MPP+诱导的PC12细胞凋亡的影响，并且探讨其作用机理。方法 采用MTT法检测细胞存活率，碘化丙啶染色流式细胞术(FCM)检测PC12细胞凋亡，罗丹明123染色FCM检测细胞线粒体膜电位（△Ψm），双氢罗丹明123染色FCM检测细胞内活性氧（ROS）的含量。结果 不同浓度MPP+作用PC12细胞24h后，细胞存活率显著下降(P0.01)，与单纯MPP+处理组比较，20μmol/L Cur和不同浓度的MPP+同时处理PC12细胞24h后，细胞存活率显著升高(P0.01)；一定浓度的Cur对PC12细胞作用24h后无明显凋亡诱导作用，但可使MPP+处理组细胞凋亡率下降(P0.01)；20μmol/L Cur可抑制MPP+引起的PC12细胞△Ψm降低及细胞内ROS含量增多。结论 姜黄素可以抑制MPP+诱导的PC12细胞凋亡，其作用机理可能与维持线粒体正常膜电位，稳定线粒体功能，清除ROS有关。 【关键词】? 姜黄素；1-甲基-4-苯基-吡啶；细胞凋亡；活性氧；帕金森病 　　Abstract:? Objective? To investigate the cytopretection of curcumin (Cur) against PC12 cell apoptosis induced by 1-methyl-4-phenylpyridium (MPP+) and the? mechanisms.? Methods? A MTT assay was used to detect the cell vitality, and PI staining flow cytometry (FCM) was used to analyze the PC12 cell apoptosis, fluorescent probe of rhodamine 123 staining was used to measure the mitochondrial potential (△Ψm), dihydrohodamine 123 (DHR) staining FCM was used to detect the levels of reactive oxygen species (ROS).? Results? PC12 cells were exposed to different concentrations of MPP+ for 24 hours. The cells vitality decreased significantly (P0.01). Compared to MPP+ alone group, after treated with 20μmol/L Cur in combination with different concentrations of MPP+, the cell vitality elevated significantly (P0.01). The indicate concentrations of Cur did not induce apoptosis in PC12 cells. However, the proportion of PC12 cells apoptosis induced by MPP+ decreased significantly after treated with Cur (P0.01). 20μmol/L Cur could prevent the decrease of △Ψm and the elevation of ROS induced by MPP+.? Conclusion? Cur can inhibit the PC12 cells apoptosis induced by MPP+, its mechanism may be associated with maintenance of △Ψm? and removal ROS.???????????????????? ??? 　　Key words:? curcumin; 1-methyl-4-phenylpyridium; apoptosis; reactive oxygn species; Parkinson disease??? 　　帕金森病（Parkinson’s disease，PD）是一种常见的中枢神经系统变性疾病，形态学研究表明PD病人脑内多巴胺（Dopamine, DA）能神经元呈凋亡样改变，提示