鱼腥草素衍生物对TNFα诱导的大鼠血管平滑肌细胞Syndecan4 表达的影响.DOCVIP

临床医学论文-鱼腥草素衍生物对TNFα诱导的大鼠血管平滑肌细胞Syndecan4 表达的影响 ???????????? 作者：王峰,欧阳平,左 琦,彭文烈, 贝伟剑，张建兴,赖文岩,许顶立 【摘要】? 目的 观察一种鱼腥草素衍生物(houttuyfonate derivative, HD)对肿瘤坏死因子α(tumor necrosis factor α, TNFα)诱导的大鼠血管平滑肌细胞(vascular smooth muscle cells, VSMCs)增殖及Syndecan4 表达的影响。方法 以终浓度分别为TNFα 20 ng/mL、HD 4 μg/mL、HD 8 μg/mL、TNFα20 ng/mL+HD 4 μg/mL及TNFα 20 ng/mL+HD 8 μg/mL对体外培养的VSMCs作用24 h，并设立相关对照组，用MTS/PMS法检测VSMCs的增殖，用Western blot蛋白免疫印迹法测定Syndecan4 的表达情况。结果 与对照组相比，HD各剂量组单独应用对VSMCs的增殖无明显作用(P0.05)；HD 4 μg/mL和8 μg/mL可分别抑制TNFα对VSMCs的增殖作用(P0.05)。Western blot测定显示：与对照组相比，HD各剂量组单独应用对Syndecan4 的表达无明显影响(P0.05)，HD 4 μg/mL和8 μg/mL均可抑制TNFα刺激后Syndecan4 的表达(P0.05)。结论 HD对TNFα刺激后的VSMCs的增殖及Syndecan4 表达均有抑制作用。 【关键词】? Syndecan4 ；鱼腥草素衍生物；TNFα；血管平滑肌细胞；细胞增殖 Abstract:Objective To investigate the effects of a new houttuyfonate derivative (HD) on proliferation of VSMCs and expression of Syndecan4? induced by TNFα in vitro. Methods Rat VSMCs were cultured and exposed to TNFα or HD respectively, and then cotreated with TNFα and HD. All the groups were cultured for 24 hours in vitro, in addition to the untreated control group established for comparison. The ratio of proliferation of VSMCs was determined by nonradioactive MTS/PMS assay and the expression of Syndecan4? was evaluated by western blot using antiSyndecan4? antibody. Results Statistical analysis showed that, compared to the control group, HD alone had no effect on VSMCs growth, but significantly inhibited VSMCs proliferation induced by TNFα. It also showed that compared to control group, HD alone had no effect on the expression of Syndecan4， but significantly inhibited the expression induced by TNFα (P0.05). Conclusions HD can inhibit proliferation of rat VSMCs and expression of syndean4 protein induced by TNFα in vitro. ??? Key words:Syndecan4 ; new houttuyfonate derivative; TNFα; vascular smooth muscle cells; cell proliferation ???? 血管平滑肌细胞(vascular smooth muscle cells, VSMCs)增殖和表型改变是心脑血管动脉粥样硬化和支架内再狭窄的主要病理基础之一,动脉粥样硬化是一个对血管损伤的过度炎