乙酰半胱氨酸对急性肺损伤的防治作用.docVIP

乙酰半胱氨酸对急性肺损伤的防治作用 【关键词】 急性肺损伤 关键词: 急性肺损伤;乙酰半胱氨酸 摘 要:目的 观察乙酰半胱氨酸对急性肺损伤的防治作用. 方法 大耳白兔24只，随机分为对照组（n=8），致伤组（n=8），乙酰半胱氨酸治疗组（n=8）.致伤组经颈静脉注入脂多糖（LPS，1mL#12539;kg-1 ）;对照组经颈静脉注入生理盐水（1mL#12539;kg-1 ）;治疗组经颈静脉在注入脂多糖（LPS，1mL#12539;kg-1 ）前2min，经颈静脉注入乙酰半胱氨酸（300mg#12539;kg-1 ）.测定各组肺动脉压、动脉血氧分压、外周血白细胞计数、肺泡灌洗液NO2- 浓度、肺系数（肺湿质量/体质量）及肺湿/干比. 结果 治疗组肺动脉压及NO2- 浓度与致伤组有显著差别（Plt;0.01），氧分压较致伤组明显改善（Plt;0.01），肺系数和肺湿/干比显著低于致伤组（Plt;0.01），外周血白细胞高于致伤组（Plt;0.01）. 结论 乙酰半胱氨酸对急性肺损伤有一定的防治作用. 　　Keywords:acute lung injury;N-acetyl-L-cysteine 　　Abstract:AIM To observe the preventive effect of N-acetyl-L-cysteine against acute lung injury.METHODS 24New Zealand rabbits were randomly divided into three groups:control group（n=8），injury group（n=8），treat-ment group（n=8）.The injury group were endotoxin（1mL#12539;kg-1 ）;the control group were given injection of normal saline（1mL#12539;kg-1 ），and the treatment group were given N-acetyl-L-cysteine（300mg#12539;kg-1 ）2minutes before the injec-tion of endotoxin（1mL#12539;kg-1 ）.Arterial oxygen pressure（PaO2 ），pulmonary arterial pressure，the count of peripher-al-blood leukocytes，the NO2- concentration in the BALF，lung index（wet weight/body weight），lung’s wet/dry weight ratio（W/D）were observed.RESULTS The pul-monary arterial pressure and NO2- concentration of the treat-ment group had significant difference with the injury group（Plt;0.01），PaO2 of the treatment group significantly im-proved（Plt;0.01）.The lung index and W/D ratio of the treatment group were significantly lower than those of the in-jury group（Plt;0.01），and the count of peripheral-blood leukocytes of the treatment group was higher than that of the injury group（Plt;0.01）.CONCLUSION N-acetyl-L-cys-teine has preventive effect against acute lung injury. 　　0 引言 　　急性呼吸窘迫综合征（acute respiratory distress syndrome，ARDS）发病机制错综复杂，急性肺损伤是其基础病变，迄今对其发病机制尚未完全阐明.研究表明炎症反应起关键作用，许多炎症介质如IL-8［1］ ，NF-Kβ［2，3］ 等参与其中.目前认为氧自由基［4］ 及氧化-抗氧化失衡［5］ 在ARDS发病中起重要作用.乙酰半胱氨酸是一种有效的抗氧化剂，能对抗氧自由基造成的肺微血管通透性增