小儿白血病干细胞测定及分离、表型特征研究.docVIP

小儿白血病干细胞测定及分离、表型特征研究 王琴 海力其古丽·努日丁 严媚① 新疆医科大学第一附属医院，乌鲁木齐市830054 【摘要】目的:测定及分离维、汉小儿白血病干细胞（leukemia stemcells,LSC），观察小儿白血病干细胞的表达水平及其免疫表型特征，探讨其表达水平及免疫表型在两个民族之间是否有差异方法:用流式细胞仪测定维吾尔族及汉族患儿LSC表达水平，其中选择部分患儿分选其LSC，体外培养观察其形态学特征并再次通过流式细胞仪检测两个民族LSC新的免疫表型，通过统计学方法进行两个民族CD34+CD38-CD123+细胞表达水平之间是否有差异结果：AML LSC CD34+CD38-CD123+细胞（P=0.04）及ALL CD34+CD7-细胞（P=0.001）表达水平在维、汉两个民族之间存在差异。培养的LSC呈集落样悬浮生长，在体外能存活8个月以上并保持细胞活性，所检测到的表面标记有：AML LSC：D34 ,CD123 ,CD96 ,CD44 ,CD33 ,CD90 ，ALL-LSC：D34 CD19 ,CD9 ,CD90。CD123+，CD90+在急淋及急非淋LSC中均有不同程度的表达，结论：AMLCD34+CD38-CD123+细胞表达水平明显低于成人，其维吾尔族及汉族白血病患儿存在差异，ALL LSC 除表达与文献相似的免疫表型外，还表达CD34+CD9+，CD34+CD19+等，这些可能是小儿LSC新的免疫表型；CD123+，CD90+可能在急淋及急非淋LSC中均存在. 【关键词】小儿；白血病干细胞；测定；分离 ；免疫表型 Determination andSeparation,Phenotypic Characterization Research of Children Leukaemia Stem Cell Objective: Determine the level of The leukaemia stem cells (LSC) of Xinjiang Children with acute leukemia, analys its immune phenotypes, karyotype, and discuss the level of The (LSC)whether there are differences between children and adult ,whether there is a new immune phenotype of children LSC and whether there is a corelation between karyotype. Methods: Determine the level of The via flow cytometer, and isolate that, via flow cytometer, observe morphology via in vitro culture, again through the flow cytometric detection new immune phenotype of LSC. Results: There is diferentation between two nation for the level of AML CD34+CD38-CD123+ cell（P=0.04） and ALL CD34+CD7-cell （P=0.001）.The cells growing both suspended and agminated mode could survived over 8 months and proliferated constantly in vitro. Detect surface markers are: Acute myeloid leukaemia stem cells (AML LSC) CD34, CD123, CD96, CD44, CD33, Acute lymphocytic leukemia stem cells (ALL-LSC) CD34, CD19, CD9.,CD90.AML Conclution: Xinjiang children LSC has similar immune phenotype of literature, olso found the CD9 +CD34 + , CD19+CD34 + etcspecial phenotype , Key Words: leukaemia children; leukaemia ste