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目 录
中文摘要 ···················································1
英文摘要 ···················································3
引 言 ····················································5
中英文缩略词对照表 ·······································6
正 文 ····················································7
1.实验材料和方法 ·········································7
1.1 实验动物及饲养条件 ··································7
1.2 主要试剂与药品 ·······································7
1.3 主要实验器材 ·········································7
1．4实验方法 ···············································8
1.4.1动物模型建立与分组 ·································8
1.4.2实验过程 ·············································8
1.4.3观察指标及检测方法 ·································8
1.4.4试剂盒检测方法 ······································9
1.4.4.1 关节液 NO 含量测定 ································9
1.4.4.2 滑膜组织 iNOS 活力测定 ·························11
1.4.4.3 蛋白定量（双缩脲法）测定 ························12
1.5 统计学处理 ············································13
1.6 主要技术路线 ·········································13
2. 实验结果 ···············································15
2.1.大体肉眼观察 ········································15
2.2 关节液 NO 含量的测定 ·································15
2.3 滑膜一氧化氮合酶的测定 ····························16
3.讨论 ····················································16
3.1 骨性关节炎动物模型的建立、选择与评价 ············16
3.2 祖国医学对膝骨性关节炎的病因病机认识 ···········19
3.3 NO 与骨性关节炎 ······································24
3.4 壮 医药治疗膝骨性关节炎的优势 ······················32
3.6.壮药骨痹方烫疗治疗膝骨性关节炎的作用机理探讨 ·33
4.存在的问题和展望 ······································34
结论 ·······················································36
参考文献 ··················································37
综 述 ··················································42
致 谢 ··················································50
攻读学位期间发表的学术论文目录 ·······················51
作者声明 ··················································52
个人简历 ·····································
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