罗格列酮对高糖诱导的血管内皮细胞炎症的抑制作用.pdfVIP

C 431262/R 2010 18 4 265 [] 2010) 1804026504 罗格列酮对高糖诱导的血管内皮细胞炎症的抑制作用 1 2 1 , , ( 1.南方医科大学珠江医院肾内科, 广东省广州市 510282; 2. 郑州大学第一附属医院肾内科, 河南省郑州市 450052) [] 糖尿病; 罗格列酮; 血管内 细胞; 内 功能失常 [ ] 阐明罗格列酮对糖尿病血管并发症保护作用的分子机制 用凝胶迁移分析和免疫荧光 法测定不同浓度罗格列酮干预前后高糖诱导的血管内 细胞核因子 B激活和血管细胞黏附分子 1表达的改变 25 mmol/L D葡萄糖刺激 30 m in 可诱导细胞核因子 B 向核内迁移(与基础水平相比, P 0. 001); 525 mol/L罗格列酮以剂量依赖的方式抑制了 D葡萄糖诱导这种效应, 抑制率分别为 25. 17% (P = 0. 001)和51. 79% (P 0. 001)罗格列酮还抑制了 D葡萄糖诱导的血管细胞黏附分子 1高表达 罗格列酮通过抑制血管内 细胞炎症起到直接保护血管作用, 可能是其延缓和改善糖尿病血管并发症的机制之一 [] R96 [] A Rosiglitazone PreventsHigh GlucoseInduced Inflammation in Vascular Endothelial Cells 1 2 1 IU HongX in, LIU ZhangSuo, and LongHaiBo ( 1. Department of N ephrology, Zhuj iangH osp ital, SouthernM edical University, uangzhou, uangdong 510282, China; 2. D epartment of N ephrology, theFirstAff iliated H osp ita,l Zhengzhou University, Zhengzhou, H enan 450052, China) [KEYWOR S] Diabetes; Rosiglitazone; Vascular EndothelialCells; EndothelialDysfunction [ABSTRACT] Aim To clarify themolecularmechanism of protective effectof rosiglitazone on vascular complication ofdiabetes. Methods Human umbilical vascular endothelialcells (HUVEC) were exposed to medium or high glucose in the presence or absence of different doses of rosiglitazone. uclear translocation of nuclear factorB( FB) was measured using the electrophoreticmobility shift assay and mi munofluorescence. Expression ofvascular celladhesionmol ecule1(VCAM1) was determ ined by mi munofluorescence. Results 25 mmol/L Dglucose significantly induced nu clear translocation of FB inHUVEC (P 0. 001 vs tha