青枯雷尔氏菌生防芽胞杆菌解淀粉芽胞杆菌的高通量筛选及其.docVIP

PAGE \* MERGEFORMAT 1 青枯雷尔氏菌生防芽胞杆菌解淀粉芽胞杆菌的高通量筛选及其鉴定项目资助：948 ；863 ；863 .通讯作者：刘波，研究员。E-mail:liubofaas@163.com. 项目资助：948 ；863 ；863 . 通讯作者：刘波，研究员。E-mail:liubofaas@163.com. 第一作者：刘国红，女，1983年出生，主要从事芽胞杆菌分类及其功能研究。E-mail:liuguohong624@. 收稿日期： ；回复日期：。 刘国红1，2，刘波1，林乃铨2 （1 福建省农业科学院农业生物资源研究所，福州 350003；2 福建农林大学，福州 350002） 摘要：芽胞杆菌具有较广的抗菌谱，对多种病原真菌和细菌均有强烈的抑制作用，具有较高的潜在生防利用价值。本研究首次采用高通量筛选法从莆田和长泰采集回的样本进行土样分离，初筛出36个出现抑菌圈的菌落，通过分离纯化和筛选，得到一株对青枯雷尔氏菌具有强拮抗作用的芽胞杆菌。通过打孔法和滤纸片法得出打孔法更适合抑菌作用研究。经过形态特征、生理生化特征和分子特征鉴定结果表明，生防芽胞杆菌B为解淀粉芽胞杆菌。此菌株的脂肪酸主要类型为15:0 iso（23.56%）和15:0 anteiso（38.13%）。本研究结果为青枯雷尔氏菌的防治提供了新的研究基础。 关键词：青枯雷尔氏菌；生物芽胞杆菌；高通量筛选；鉴定 题目，，，，， Abstract: Genus Bacillus has a wider antibacterial spectrum, and a strong inhibitory effect on a variety of pathogenic fungi and bacteria, having a high potential biocontrol value. It was the first reported that high-throughput screening method was used to screening biocontrol Baciilus species. In this study, we collected 36 biocontrol strains from Putian and Changtai soil samples, after purification and screening, one Bacillus strain was obtained that had a strong antagonistic effect. Drilling method and the filter paper method were used to study inhibition, finding drlilling method was more suitable for the antimicrobial effect. Morphological characteristics, physiological and biochemical characteristics and molecular characterization results showed that the biocontrol Bacillus strain was Bacillus amyloliquefaciens. The main fatty acid types of this strain was the 15:0 iso (23.56%) and 15:0 anteiso (38.13%). The results of this study provides a new research base for the prevention and control of Ralstonia solanacearum. Keywords: Ralstonia solanacearum; Biocontrol Bacillus species; high through screening; identification 青枯菌雷尔氏菌 (Ralstonia solanacearum)是一种革兰氏阴性，杆状，广泛分布于热带、亚热带地区，引起包括作物与林木毁灭性青枯病的病原菌(Hayward,1994)。青枯病菌过去分类上一直属假单胞杆菌属(Pseudomonas)，Yabuuchi等(1992)根据该菌16S rRNA序列、DNA一DNA比较同源性、脂肪酸组成等性状，将其置于布克氏菌属(Burkholderia )，后来通过系统发育与多相表型分析又发现其不同于布克氏