猪5个群体SLA微卫星遗传多样性.pdfVIP

HEREDITAS (Beijing) 2012 11 , 34(11): 1427―1433 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2012.01427 猪5 个群体SLA 微卫星遗传多样性 1 1, 4 1 1 2 3 于辉 , 刘荣辉 , 李华 , 左启祯 , 李岩 , 吴珍芳 1. , 528231; 2. , 511700; 3. , 510642; 4. , 405400 文章旨在比较广东地方猪、华南野猪(S.s. chirodontus) 和引入品种白细胞抗原复合体(Swine leukocyte antigen complex, SLA) 的遗传多样性, 为猪的抗病选育提供理论依据。在SLA 区域内选取18 个多态性丰富的微 卫星(SLA-MS), 分别对皮特兰、杜洛克、大花白、蓝塘猪以及华南野猪进行遗传分型。结果表明, SLA 不同区 域平均遗传多样性高低依次为SLA Ⅱ(He=0.628, PIC=0.581) SLA Ⅰ(He=0.530, PIC=0.474)SLA Ⅲ (He=0.526, PIC=0.458); 5 个群体间分子多样性指数(MDI)依次为华南野猪(0.716) 蓝塘猪(0.614) 大花白(0.559)皮特兰 (0.550)杜洛克(0.507) 。总体看来, SLA-MS 的遗传多样性高低依次为华南野猪广东地方猪 引入品种。基于 Garza-Williamson 指数(GWI)分析, 杜洛克和大花白相较于华南野猪瓶颈效应严重; 遗传距离分析表明蓝塘猪 和华南野猪首先聚为一类, 然后与大花白聚为一大类, 而皮特兰和杜洛克则单独聚为一支。文章为广东地方猪 种质资源的保护、抗病选育和配套系的生产提供了理论基础。 猪白细胞抗原复合体; 微卫星; 遗传多样性 Genetic diversity based on swine leukocyte antigen complex mi- crosatellites(SLA-MS) in five pig populations YU Hui 1, LIU Rong-Hui 1,4, LI Hua 1, ZUO Qi-Zhen 1, LI Yan 2 , WU Zhen-Fang 3 1. Department of Animal Science, Foshan University, Nanhai 528231, China; 2. Dongguan Institute of Animal Science, Dongguan 511700, China; 3. College of Animal Science and Technology of South China Agricultural University, Guangzhou 510642, China; 4. The Kai Xian Bureau of Husbandry and Veterinary, Chongqing 405400, China Abstract: The genetic diversity of swine leukocyte antigen complex (SLA) was studied among Guangdong local pigs, Huanan wild boars (S.s. chirodontus) and introduced pigs, which aimed at providing a theoretical foundation for further pig anti-disease resistance breeding. Pietrain pigs, Duroc pigs, Large black-white pigs, Lantang pigs, and Huana