猪CAST基因多态性与肌纤维组织学特性及屠宰性状的相关性分析.pdfVIP

HEREDITAS (Beijing) 2007 1 , 29(1): 65―69 ISSN 0253-9772 研究报告 DOI: 10.1360/yc-007-0065 CAST 武艳群, 吴旧生, 赵晓枫, 郭晓令, 徐宁迎 , 310029 : 采用3 种限制性核酸内切酶对45 头金皮(金华×皮特兰)F 代猪钙蛋白酶抑制蛋白基因(CAST) 的第6 外显 2 子和第7 外显子之间的片段进行了PCR-RFLP分析, 结果显示在第6 内含子内均检测到Msp Ⅰ、Hinf Ⅰ和Rsa Ⅰ酶 切多态性。根据酶切结果将CAST基因分为 3 种基因型(AACCEE 、BBDDFF 、ABCDEF ), 其基因型频率分别为 0.1778, 0.2222, 0.6000 。对背最长肌做连续性石蜡切片, 分别采用苏木精-伊红和肌球蛋白重链免疫组织化学方 法染色并拍照, ImageJ软件统计肌纤维横截面积、密度、直径及MHC Ⅰ型肌纤维的比例。采用SPSS程序分析了 CAST不同基因型对金皮F2代猪肌纤维组织学特性和屠宰性状的影响。结果表明: BBDDFF基因型个体的肌纤维 横截面积和眼肌面积显著地高于ABCDEF 基因型个体的肌纤维横截面积和眼肌面积(P 0.05) 。 : 金皮F 代猪; CAST基因; 肌纤维; 免疫组织化学 2 Correlation between porcine CAST gene polymorphism with muscle fiber histological traits and carcass characteristics WU Yan-Qun, WU Jiu-Sheng, ZHAO Xiao-Feng, GUO Xiao-Ling, XU Ning-Ying College of Animal Science, Zhejiang University, Hangzhou 310029, China Abstract: Three polymorphisms were identified in the sixth intron of the CAST gene by PCR-RFLP using enzymes Msp Ⅰ, Hinf Ⅰ and Rsa Ⅰ in 45 Jinpi F2 pigs. However, only three genotypes AACCEE, BBDDFF , and ABCDEF were detected in those pigs and the genotype frequencies were 0.1778, 0.2222 and 0.6000, respectively. Longissimus dorsi muscles were moved for paraffin serial sections and stained with hematoxylin-eosin or myosin heavy chain (MHC) by immunohisto- chemistry respectively. Fiber cross-sectional area , fiber density, fiber diameter, the rate of MHC type Ⅰfiber and the carcass characteristics were recorded. Correlation analysis showed that the skeletal muscle fiber area and the eye muscle area of the BBDDFF individual were significantly