siRNA沉默HIF-1α对缺氧培养的人脉络膜黑色素瘤细胞EPO表达的影响.pdfVIP

lUlI l UlU l I I III IIIII Y2338237 硕士研究生：孟凡君(眼科学) 导师：周占宇副教授 关键词：脉络膜黑色素瘤；缺氧诱导因子一1Q；促红细胞生成素； 小片段干扰RNA；RNA干扰 Effectof HIF．1仅 siRNAon Silencingby of Expression In ErythropoietinMelanomaofthe Malignant ChoroidCell under Hypoxia Abstract Objectives：Tothe of investigateexpression inducible hypoxia factor一1aand in erythropoietin melanomaof malignant thechoroidcellline OCM．1 under meansof hypoxia small by interferenceRNA transferring withcationic liposome． Inordertoobserve theeffectof HIF一1O【in melanomaof malignant thechoroid． Methods： OCM一1cellof normoxicwere culturedonceii group cuItureflask 1n concentrationof high DMEM glucose 1 OOU／ml containing0％FBS，l penicillin． 100 the gg／ml cellsare streptomycin，andmaintainedat37℃inahumidified5％ incubator．We C02 tookthecellin