HPV16E6E7基因修饰树突状细胞疫苗诱导CTL致CaSki细胞凋亡体外实验研究.pdfVIP

∀ HPV 16 E6E7 CTL CaSki ∀ 2 ! 115 ! doi: 10. 3969/ j. issn. 1000484X. 2011. 02. 005 !! HPV 16 E6E7 CTL CaSki ∀ ∀ ∀ ∀ ∀ ∀ ∀ ∀ ∀ ∀ ∀ ( 重庆医科大学分子医学与肿瘤研究中心, 重庆400016) ∀ ∀ ∀ R392. 11∀ R737. 33 ∀ ∀ ∀ A∀ ∀ ∀ 1000484X(2011 [ ∀ ] ∀ : 16(HPV16) E6E7 ( pAdE6E7) ( Dendriti ell, DC) , DC T ( Cytotoxi T lympho yte, CTL) CaSki : pAdE6E7 DC , , ( CD CD MHC# CD ) DC T 40 86 11C , CaSki , DAPITUNEL CaSki : pAdE6E7 , 40% ~ 50% , HPV 16 E6E7 , T , DAPITUNEL CaSki : HPV 16 E6E7 DC DC , CTL CaSki [ ] ∀ ; T ; CaSki ; Const uction of genetically modified dend itic cell vaccine exp essing HPV16 E6E7 fusion gene and activating specitific cytotoxic T lymphocytes to induce apoptosis CaSki cells in vit o JIA O QingFang, YI FaPing, WANG Chang Dong, YUAN ChengFu, LI U Le, LAN Huan, F U ShaoYue, AI Qing, SONG FangZhou. Molecula Medicine and Cance Resea ch Cente , Chongqing 400016, China [Abst act] ∀ Objective:To develop the re ombinant adenovirus arrying HPV16 E6E7 ( pAdE6E7) to infe t mouse immature dendriti ells, and to observe the apoptosis of CaSki ells indu ed by spe ifi ytotoxi T lympho ytes ( CTL) triggered by geneti ally modified DC va ine expressing HPV16 E6E7 fusion gene in vitro. Methods:The pAdE6E7 was used to infe t mouse immature dendriti ells to prepare DC va ine, the onfo al mi ros opy was used to observe the expression of green fluores en e protein in immature dendriti ells, and flow ytome try ( FCM) was used to examine the surfa e expression of CD , CD , MHC # and CD in both uninfe ted and infe ted dendriti ells. DC 40 86 11C va ine was used to indu e spe ifi CTL , an