一个可在板栗疫病菌中自主复制的穿梭表达载体.pdf

一个可在板栗疫病菌中自主复制的穿梭表达载体 摘要 本实验窒2003颦首次构建了可在板梁疫病菌中囱主复制囟勺穿梭质粒 制驳及穿梭嚣关的多余琦段。将最终获褥的不链继续缩《、酌质粒上的rrrc启动 予和终止予鬣换为板巢疫病菌gpd启动子与终止子，并在gpd启动予与终止子 之蠢添麴7多克隆位纛，将大小约必15。6 kbp 檄栗疫病菌细胞内自主复制。携带漆酶基因LAC-I 鼙组质粒都研以在扳票疫病菌低毒菌株EP713中高效表达出漆酶活性，表明 疫病菌细胞质内运动的能力。穿梭表达载体pFC9的成功构建为板粱疫病菌的 萋因功麓磺究提供了一个寮赣酶王具。 关键诿：童童复制蓑粒摄粟疫痰薅窭援表达载傣鏊丝敲合pFC9 ofAShuttie Development Vector,pFC9，for Expression parasitica A8STRAe羊 have a we There been few ofshuttle for filamentous only examples plasmids fungi．Here theconstructionofashuttle chestnut blight report vector,pFC9，for fungus， expression Cryphonectria froma found is parasitica，developedpreviously to in wasabout8．9 in able autonomously e kbp size，its replicateparasitica．pFC9 sitesflankedwith andterminator with multi—cloning gpd promoter as oflaccasewasachieved resistancesdcctable levelof marker．High hygromycin expression the in of harbored thetransformants C strain．EP713，which h