毛细管电泳法测定中药萹蓄中槲皮素、山柰素和咖啡酸.docVIP

毛细管电泳法测定中药萹蓄中山柰素、槲皮素和咖啡酸 冯海燕1，李向军2，王惠1，任蕾1，牛金玉1 （1.石家庄学院 化工学院，石家庄 050035；中国科学院研究生院 化学学院，北京 100049） pH 8.5的20 mmol·L-1 Na2B4O7-NaH2PO4为缓冲溶液，运行电压20 kV，重力进样时间14 s（10 cm高度差），在254 nm波长处紫外检测。山柰素、槲皮素和咖啡酸在7 min内即可实现分离，线性范围依次为0.50~100，0.70~146，1.0~124 mg·L-1，检出限（3S/N）依次为0.3，0.4，0.6 mg·L -1。采用该方法对萹蓄样品中3种分析物的含量进行了测定，加标回收率在95.1%~106.0%之间。 关键词：毛细管电泳法；萹蓄；山柰素；槲皮素；咖啡酸 中图分类号：O657.1 文献标识码：A 文章编号： Determination of Kaempferol, Quercetin and Caffeic Acid in Polygonum Aviculare L by Capillary Electrophoresis FENG Hai-yan1，LI Xiang-jun2，WANG Hui1，REN Lei1，NIU Jin-yu1 (1.School of Chemical Engineering, Shijiazhuang University, Shijiazhuang 050035，China; 2. College of Chemistry and Chemical Engineering, Graduate University of the Chinese Academy of Sciences, Beijing 100049,China) Abstract：A method based on capillary electrophoresis was proposed for the determination of kaempferol, quercetin and caffeic acid in Polygonum aviculare L. Effects of several important factors such as the pH and concentration of buffer, sample injection time were investigated to optimize the conditions. The analytes could be well separated within 7 min by using 20 mmol·L-1 Na2B4O7-NaH2PO4 (pH 8.5) as the buffer solution at a working voltage of 20 kV. Samples were injected by a gravity injection method with 10 cm hydrostatic pressure for 14 s. UV detection was performed at 254 nm. Linearity ranges of 0.50~100, 0.70~146 and 1.0~124 mg·L-1 were obtained for kaempferol, quercetin and caffeic acid respectively with the detection limits (3S/N) of 0.3, 0.4 and 0.6 mg·L-1. The method has been applied to the analysis of these analytes in Polygonum aviculare L, and the values of recovery were found in the range from 95.1% to 106.0% according to standard addition method. Keywords: Capillary electrophoresis；Polygonum aviculare L；Kaempferol；Quercetin；Caffeic acid 萹蓄为蓼科蓼属植物萹蓄Polygonum aviculare L.的干燥全草，为《中国药典》2000年[1]。近年研究发现萹蓄还具有良好的抗癌活性[2]。中药药性的发挥和其化学成分，[3-5]，该方法多以有机溶剂作流动相，样品消耗量大, 且分析时间一般较长；萹蓄中咖啡酸的测定方法目前