低压缺氧对大鼠脑线粒体腺苷酸转运体特性的影响.pdfVIP

生理学报 Acta Physiologica Sinica, February 25, 2006, 58 (1): 29-33 29 研究论文 低压缺氧对大鼠脑线粒体腺苷酸转运体特性的影响 * 陈丽芬，柳君泽 ，李 兵 第三军医大学高原军事医学系病理生理学与高原生理学教研室，全军高原生理和高原病研究重点实验室, 重庆 400038 摘 要：本文探讨低压缺氧对大鼠脑线粒体内膜腺苷酸转运体(adenine nucleotide translocator, ANT)转运特性的影响。实验 将雄性Wistar 大鼠随机分为常氧对照组和缺氧组，后者分别连续暴露于模拟5 000 m 高原1、5 、15 、30 d (23 h/d) 。分 别于平原和模拟4 000 m 高原断头处死动物，分离脑线粒体，用抑制剂终止法测定线粒体对3H-ADP 的转运效率，抑制剂 滴定法测定ANT 密度，HPLC 测定线粒体内腺苷酸含量。结果显示：缺氧后ANT 转运活性均明显低于常氧组，缺氧不 同天数线粒体内膜ANT 分布密度无显著改变，线粒体内(ATP+ADP)含量下降与转运活性变化一致。以上观察结果表明， 低压缺氧暴露可显著抑制ANT 转运活性，降低能量产生和利用的周转率，但不改变ANT 密度，提示ANT 活性改变是低 压缺氧时细胞能量代谢障碍的重要机制。 关键词：缺氧；线粒体；腺苷酸转运体 中图分类号：Q 42 6 ；R 33 8. 2 Characteristics of adenine nucleotide translocator in mitochondria of rat cerebral cortex during hypobaric hypoxia exposure * CHEN Li-Fen，LIU Jun-Ze ，LI Bing Department of Pathophysiology, College of High A ltitude Military Medicine, the Third Military Medical University, Chongqing 400038, China Abstract ：The purpose of the present study was to explore the effects of hypoxic exposure on mitochondrial adenine nucleotide translocator (ANT) activity and its characteristics. Male Wistar rats were exposed to hypoxia in a hypobaric chamber simulating high altitude at 5 000 m for 1, 5, 15 and 30 d. Control rats were fed outside the hypobaric chamber. Rats were sacrificed by decapitation and mitochondria from the cerebral cortex were isolated by differential centrifugation at each time point. The ANT activity was detected by 3 the atractyloside (ATR)-inhibitor stop technique. Mitochondria was initiated by addition of H-ADP and terminated after 12 s by quick 3 addition of ATR. The radioactivity was measured in a li