分子生物学实验 - 韩山师范学院.docVIP

分子生物学实验 (基因工程部分) 韩山师范学院生物系分子生物学实验室 2007.3.1 目 录 分子生物学实验综合规程·······················································································3 参考书目 ·················································································································4 实验内容···················································································································4 实验一 实验基础知识和基本技能培训·································································5 实验二 大肠杆菌感受态细胞的制备·····································································5 实验三 质粒DNA的转化·······················································································6 实验四 碱裂解法提取质粒DNA············································································7 实验五 琼脂糖凝胶电泳检测DNA·····································································10 实验六 PCR基因扩增··························································································12 实验七 DNA重组（一）－DNA的酶切································································14 实验八 DNA重组（二）－DNA片段回收和连接················································14 实验九 DNA重组（三）－重组DNA的转化························································14 实验十 植物基因组DNA的提取·········································································17 实验十一 脯乳动物基因组DNA的制备·····························································19 实验十二 Sourthern 杂交·····················································································21 实验十三 外源基因在大肠杆菌中的表达······························································24 实验十四 DNA序列测定·····················································································27 分子生物学实验综合规程 上课时间提前5分钟进入实验室；不迟到、不早退，无故旷课累计3次没成绩； 请假须医院病假条或班主任签字的假条； 上课时需穿实验服，不许在实验室内吃东西，不背着书包做实验，不穿拖鞋进实验室； 实验课期间不许打闹、闲聊等，不大声喧哗，保持课堂纪律，实验过程中，至始至终需全组同学一起完成，任何人中途不得缺席。 必须做到课前认真预习，课间做好实验记录、课后详细总结实验结果并按时提交实验报告； 公用试剂不得污染！每次都必须用新枪头。公用台上公用试剂不得私自拿到自己实验台上； 不得私自拿走或乱放别人的实验物品、实验产品； 不得擅自使用实验室中非本实验仪器、试剂。 实验废液不随便倾倒，随时保持实验台的整洁； 爱护实验器材，实验器材损坏必须按规定赔偿； 实验前按仪器性能与要求