雄激素与体外培养的人毛乳头细胞和外根鞘细胞相互作用的研究.pdfVIP

232 2006 6 17 3 Chinese J Prac A es h Plas Surg , Jun 2006. Vol. 17 N o . 3 刘 昕, 胡志奇, 刘晓燕, 吴 越, 官 浩 ! ( ) , , , , , ( P 0 . 05) 10- 9 ml/ L ( P 0 . 05) 10- 8 ml/ L 10- 7 ml/ L ( P 0. 01) , 10- 10 ml/ L ( P 0 . 05) 10- 9 ml/ L 10- 8 ml/ L 10- 7 ml/ L ( P 0. 0 1) , , , ! ; ; !R329. 2 !A !1672- 7290( 2006) 03- 0232- 04 Research of interaction between human dermal papilla cells and outer root sheath cells cultured in vitro with the androgen (testosterone) LIUXin, H U Zhi- qi, LI UXiao- yan, et al. (Department of Plastic Surgery, Nanf ang Hosp ital, Southern edical University , Guangzhou 5105 15, China) Abstract: Objective T o obser ve he prolif era ive ac iv i y of human dermal papilla cells and ou er r oo shea h cells in vi ro and he resul s of he in erac ion w i h androgen on diff eren concen ra ion . Methods Der mal papillas w er e isola ed from bald and nonbald adul s scalp w i h he me hod of dissec ion by collage nase diges ion , hen he dermal papilla cells w ere cul ured in vi ro w i h kera ino cy e cells medium w i hou serum . Ou roo shea h cells from nonbald adul s w er e cul ured in v i ro scalp. T he es os erone o n diff eren concen r a ion w as added in he ou er roo shea h cells w hich w as cocul ur ed w i h he dermal papilla cells from he bald and nonbald adul scalp and hen he prolif er a ive ac ivo y o f he ou er roo shea h cells w er e ex amined. Results T es os erone show ed no eff ec s on prolif era ion of ei her dermal papilla cells or ou er r oo shea h cells cul ured alone ( P 0 . 05 versu s con ro l) . 10- 9 ml/ L concen r a ion of es os erone ( P 0. 05 versus con rol) 10- 8 ml/ L and 10- 7 ml/ L concen r a io n of es os er one( P 0. 01 v ersus con rol) s imula ed prolif er a ion of