电针对非酒精性脂肪肝大鼠下丘脑瘦素及瘦素受体表达的影响.pdfVIP

2010 8 35 4 # 277 # 白春艳 卓廉士 朱 艺 傅 燕 ( 重庆医科大学中医药学院, 重庆400016) : 探讨电针治疗非酒精性脂肪肝( NA LD) 的作用机制: 将32 只SD 大 随机分为模 型组22 只及空白组10 只模型组喂养高脂饲料制备NA LD模型8 周后将模型组大 随机分为对照组 11 只及针刺组11 只针刺组分别电针! 丰隆∀! 足三里∀! 太冲∀治疗4 周后检测各组血清甘油三酯( TG) 血清胆固醇(T C) , 观察肝组织病理学变化, 用逆转录酶链聚合反应检测下丘脑组织中瘦素( Lep) 及其受体 mRNA 的表达: 造模后空白组与模型组比较, 大 T CT G 显著升高( P 001) , 肝组织出现弥漫性 脂肪变电针4 周后, 电针组与对照组比较, 大 T CT G 显著下降, 肝组织脂肪变减轻, 下丘脑Lep 和瘦素 受体mRNA 表达升高( P 005) : 电针对NA LD 大 有较好的治疗作用, 其作用机制可能是通过 调节下丘脑瘦素及其受体 mRNA 的表达实现的 电针 非酒精性脂肪肝 下丘脑 瘦素 瘦素受体 + R 2459 7 A 2010) Effect of Electroacupuncture on HypothalamicLeptin and Leptin Receptor mRNAExpression in Rats with Nonalcoholic Fatty Live Disease BAI Chunyan, ZH UO Lianshi, ZHU Yi, U Yan ( Co lleg e of Ch inese M ed ic ine, Chong qing M ed ical Univ ers ity , Chong qing 4 00016 , China) ABSTRA T Objective T o observe t he effects of electroacupuncture ( EA ) on leptin and leptin receptor( Obrb) mRNA expression in t he hypot halamus of nonalcoholic fatty liver disease ( NA FLD) rats so as to reveal its underlying mechanism in t he treat ment of NAFLD inflammation. Methods Thirtytw o male SD rats were randomly divided i nto control group ( n = 10) model group ( n= 11) and EA group ( n = 11) . NAFLD model was established by feeding the ani mals wit h high fat diet for 8 weeks. EA ( 10 Hz 1 5 V 30 min) was appli ed to unilateral ! Fenglong∀ ( ST 40) and ! Zusanli∀ ( ST 36) once daily for 4 weeks. Aft er t he treat ment all the rats w ere sacrificed for collecting the liver to make pathological observat ion aft er HE stai ning and for taki ng the hypothalamus tissue out to detect t he leptin mRNA and leptin receptor ( Obrb) mRNA expression by reverse transcri ption polymerase