Erg11基因突变与白念珠菌对唑类药物耐药性的初步研究.pdfVIP

108 2007 4 20 7 2 Chin J Infect Chemother, Apr1 2007, Vol1 7, No1 2 # # Erg11 周 辉, 龙铁军 : Erg1 1 , , PCR Erg1 1 , , 2 , Erg1 1 , ; 6 , Erg1 1 E26 6DY2 57 HK2 59EA 114SF487 , A 114SY257 H K2 59EF487 Erg 11 , Erg 11 , Erg 11P , Erg 11P : ; ; :R3 79 .4 ; R978 . 5 :A : 10 09-7 70 8( 20 07) 02-0 108-03 Contribution of mutations in the cytochrome P450 lanosterol demethylase ( Erg11) gene to azole resistance in Candida albicans ZH OUH ui, L ON G Tie-j un. (M icrobiology L aboratory, Changsha Mater nity and Childrens H os i- tal , Changsha 4100 07, China) Abstract: Ob ective To determine the mechanisms of resistance to azoles by sequencing analysis of Erg11 gene in 8 isolates of Candida albicans . Methods The Erg1 1 genes of 8 resist ant C. albicans isolates from patients w ere amplified by PCR and were directly sequenced. The sequencing results w ere analyzed by B AST and compared with Erg1 1 gene of C. albicans strain B59 63 0 to find out t he locat ions of mutation. Results All mutations of Erg1 1 gene were silent in 2 susceptible isolates. How ev- er five mutations E26 6D, Y257 H , K259E, A1 14 S, and F487 were identified from the 6 resistant isolates. Y2 57 H , K259E, A1 14 S, and F487 w ere characterized as novel mutat ions. Conclusions Resistant mechanism to azole agent s is correlated w ith gene mutat ion of Erg 11, w hich results in the alteration of Erg1 1P structure, and reduces the affinity of the enzyme t o azoles. This finally leads to t he resistance to azole agents. Key words: Gene mutation; Resistance; Sequencing analysis ( Cand ida albicans ) , , , , , ( ) 6 2003 9 ) 2003