HPLC_ICP_MS法测定水样中的甲基汞_乙基汞和无机汞92419.pdfVIP

28 福建分析测试 Fujian Analysis Testing 2009 ，18（1） HPLC- ICP- MS 法测定水样中的甲基汞、乙基汞和无机汞 1 王征，游飞明，邱秀玉，黄红霞 （福建省中心检验所，福建福州350002） 摘 要：建立了高效液相色谱（HPLC）和电感耦合等离子体质谱（ICP- MS）联用测定环境水样中的甲基汞、乙基汞和 无机汞的方法。实验使用的高效液相色谱流动相为含有0. 06 mol/L乙酸氨，20μg/L Bi，0.1% （V /V ）2- 巯基乙醇的5% （V /V ）甲醇- 水溶液，色谱柱为C 反相柱（5μm, 2.1mm ×50 mm），经前处理的水样在液相色谱中分离后，进入电感 18 耦合等离子体质谱检测其甲基汞、乙基汞和无机汞的浓度。甲基汞、乙基汞和无机汞检出限分别为0. 05μg /L、0. 10μg /L和0.10 μg /L。 关键词: 高效液相色谱；电感耦合等离子体质谱；联用技术；汞；形态分析 中图分类号：O657.72 文献标识码：A 文章编号：1009- 8143(2009)01- 0028- 04 Simultaneous determination of the content of Methyl mercury ，Ethyl mercury and Inorganic mercury in water samples by HPLC-ICP-MS 1 Wang Zheng , You Feiming, Qiu Xiuyu, Huang Hongxia (Fujian Provincial Central Inspection Institute, Fuzhou, Fujian 350002, China) Abstract:A confirmatory method is presented for the determination of Methyl mercury, Ethyl mercury and inorganic mercury in environmental water samples by high performance liquid chromatography coupled with inductively coupled plasma- mass spectrometry (HPLC- ICP- MS). A 5%(V /V) methanol- water mobile phase containing 0.06 mol/L ammonium acetate, 10μg/L Bi and 0.1%(V /V ) 2- mercaptoethanol and a C18 reversed phase column (5μm, 2.1 mm ×150 mm) was used for separation of Methyl mercury, Ethyl mercury and inorganic mercury by HPLC. The concentrations of three Hg species in eluent were detected by ICP- MS. The limits of the method were about 0.05 μg /L, 0.10 μg /L and 0.10 μg /L for Methyl mercury, Ethyl m