环氧合酶2基因沉默诱导人肝癌细胞凋亡的作用观察.pdfVIP

Med J Chin PLA, Vol. 37, No. 7, July 1, 2012 715 2 2(COX-2)mRNACOX-2(COX-2 shRNA)HepG2 COX-2RNA COX-2 shRNA Lipofectamine 2000 HepG2 RT-PCR COX-2 mRNA COX-2CCK-8COX-2 shRNA Western blotting COX-2Bcl-2 2COX-2 shRNAHepG2COX-2 mRNA41.66% 77.79% G418 COX-2 shRNACOX-2 mRNA75.30% 29.33% COX-2 shRNA17.83%4.63% 4.47% G0 /G1 73.93%61.2% 57.630% S 13.43%26.03% 26.90% Western blotting COX-2 shRNAHepG2COX-231.25%( P0.01)Bcl-2 54.93%( P0.01) pSilencer 2.1-U6-COX-2 shRNA HepG2COX-2 Bcl-2 2 shRNA bc