羊栖菜多糖改善2型糖尿病大鼠胰岛素抵抗的实验研究.pdfVIP

Chin J M ar Drugs, 2011 A ug ust, Vol . 0 No. 4 2011 8 30 4 2 * 杨旭东, 张杰, 崔荣军 ( , 157011) : 探讨羊栖菜多糖( S argassumf us if orme polysaccharide, SFPS) 对2 型糖尿病( 2DM ) 大鼠胰岛 素抵抗和脂滴包被蛋白( Perilipin) 基因表达的影 Wist ar 大鼠饲喂高糖高脂饲料加链脲佐菌素 1 1 (Streptozotocin, S Z) 建立2 型糖尿病大鼠模型实验组灌胃给予SFPS 1. 6, 0. 8, 0. 4 g kg d 8 周 后, 测定FBGFINS 和血脂水平; R PCR 法检测脂滴包被蛋白基因的表达水平 与模型组比较, 试验 组FBGFINSCGLDLCFFA 均降低, 脂滴包被蛋白基因的表达增加, 差别有统计学意义 SFPS 可改善糖脂代谢紊乱和降低胰岛素抵抗, 其机制可能是提高脂滴包被蛋白基因表达 : 羊栖菜多糖; 糖尿病; 胰岛素抵抗;血脂; 脂滴包被蛋白 :R931.711, R965 : A :2011) Experimental study of Sarg assumf usif orme polysaccharides on insulin resistance in type two diabetes mellitus rats YANG Xudong, ZHANG Jie , CUI Rongjun ( Mudanj iang Med ical College , M ud anj iang 157011, China ) Abstract: Objective o investigate the effects of SFPS on the insulin resistance ( IR) and level of perilipin gene expression in type 2 diabetes mellitus(2DM) rats. Methods Wistar rats were feeded with high fatty and high sugar diet and S Z was injected to induce type 2 diabetic model. he groups were treated with 1 1 1. 6, 0. 8, 0.4 g kg d SFPS respectively by intragastric administration. After 8 weeks, the levels of fasting blood glucose ( FBG) , fasting insulin ( FINS) and blood lipids were measured, and expression of perilipin gene was determined by R PCR. Results Compared with model group, levels of FBG, FINS, C, G, LDLC, FFA were lowered and highe