银屑病患者血小板表面α-颗粒膜蛋白的放射免疫测定.x.docxVIP

银屑病患者血清中神经免疫蛋白与淋巴细胞转化的研究银屑病患者血清中神经免疫蛋白与淋巴细胞转化的研究中华皮肤科杂志 1998年第2期第31卷 论著作者：杨雪琴　邵黎　那爱华　郭秀兰　庞晓文　李世荫　范少光单位：100036 北京，空军总医院皮肤科(杨雪琴　那爱华　郭秀兰)；北京医科大学生理学系(邵黎　范少光)；北京医科大学第三医院皮肤科(庞晓文　李世荫)关键词：银屑病；神经免疫蛋白；淋巴细胞转化【摘要】　目的　为了解紧张、压抑等心理因素诱发、加重银屑病的物质基础。方法　采用放射免疫法测定了49例银屑病患者和34例正常人血清对正常小鼠淋巴细胞转化的影响；以及用神经免疫蛋白(NIP)单克隆抗体中和银屑病患者血清，然后对正常小鼠淋巴细胞转化的抑制作用的试验。结果　银屑病患者血清对正常小鼠淋巴细胞的转化率为41.55%，正常人血清对正常小鼠淋巴细胞的转化率为68.09%，表明银屑病患者血清中有抑制正常小鼠淋巴细胞转化的物质，进行期患者血清中的抑制作用大于静止期，即皮损面积愈大者其血清对小鼠淋巴细胞转化率的抑制作用愈强(P＜0.01，r=0.4939)。结论　银屑病患者血清中存在抑制淋巴细胞转化的物质，通过NIP单克隆抗体中和试验证明此物质与NIP相同或类似，但NIP与银屑病发生和发展的因果关系有待进一步研究。The Study of Neuro-immune Protein and the Lymphocyte Transformation in Psoriatic SerumYang Xueqin, Shao Li, Na Aihua, et al. Department of Dermatology, Airforce General Hospital, Beijing 100036【Abstract】　Objective　To find out the material base of the induction and aggravation of psoriasis by psycological factors such as stress and depression etc.Methods　Radioimmunoassay (RIA) was used to determine the effect of sera from 49 psoriatic patients and 34 healthy controls on the suppression of the lymphocyte transformation of normal mouse lymphocytes.Results　The lymphocyte transformation rate of mouse lymphocytes treated with the sera of psoriatic patients was 41.55%, and that of controls was 68.09%. The suppressive effect of sera in patients during the progressive stage and with more extensive lesions were stronger than that in the stable stage and with less lesions (P＜0.01, r=0.4939). The lymphocyte transformation rate was increased after adding neuro-immune protein (NIP) monoclonal antibody to the sera of patients with psoriasis.Conclusion　 The authors suggest that there is a factor which can suppress lymphocyte transformation existed in psoriatic patients serum, and it is similar to NIP, which can be neutralized by NIP monoclonal antibody, but the relationship between NIP and the pathogenesis of psoriasis remains to be studied.【Key words】　PsoriasisNeuro-immune proteinLymphocyte transformation我们曾运用诱发性规范化序列的心率变异性研究银屑病患者的植物