【大学课件】常用分子生物学技术的原理及应用.ppt

常用分子生物学技术的原理及应用 The popular technology in molecular biology: principle and application 第 一 节分子杂交与印迹技术Molecular hybridization  blotting technology （一）Southern blotting Northern blotting　结果 蛋白质的转移只有靠电转移。 蛋白质的检测以抗体作探针。 用碱性磷酸酶（ALP）、辣根过氧化酶（HRP）标记第二抗体，底物显色来检测蛋白区带的信号，底物亦可与化学发光剂结合以提高敏感度。 或用同位素标记第二抗体，放射自显影法检测蛋白区带的信号。 斑点印迹 (dot blotting) A Dot blot is a technique in molecular biology used to detect biomolecules. It represents a simplification of the northern blot, southern blot, or western blot methods. In a dot blot the biomolecules to be detected are not first separated by electrophoresis. Inst