高速逆流色谱法分离花椒中的黄酮化合物.pdfVIP

6 2013, Vol.34, No.12 1 2 2 (1. 210007 2. 210042) V V (4:3:0.5:2 / ) 850r/min2mL/min254nm6 4(ABCD)A (foeniculin) B-7-(quercetin-7-glucoside) C(rutin) D-3-(quercetin- 3-galactoside) Separation and Preparation of Flavonoids from Zanthoxylum bungeanum by High-speed Countercurrent Chromatography 1 2 2 SHI Xue-ping WU Liang-liang ZHANG Wei-ming (1. Jiangsu Food Safety Engineering Research and Development Center, College of Engineering and Technology, Jiangsu Institute of Economic and Trade Technology, Nanjing 210007, China 2. Nanjing Institute for Comprehensive Utilization of Wild Plant, Nanjing 210042, China) Abstract Flavonoids from Zanthoxylum bungeanum were separated by high-speed countercurrent chromatography. The separation was performed using a two-phase solvent system composed of chloroform, methanol, n-butanol and water V V (4:3:0.5:2, / ) at a fl ow rate of 2.0 mL/min, and rotation speed and detection wavelength were set at 850 r/min and 254 nm, respectively. Six fl avonoids were detected by HPLC. The purities of peaks 1, 2, 3, 4, 5 and 6 were 72.7%, 67.8%, 89.6%, 96.5%, 94.1% and 91.3%, respectively. Their structures were characterized as foeniculin, quercetin-7-glucoside, rutin and quercetin-3-galactoside by MS and 1H-NMR. Key words Zanthoxylum bungeanum high-speed countercurrent chromatography (HSCCC) fl avonoids separation R284.2 TS209 A 1002-6630(2013)12-0006-05 doi:10.7506/s