三种抗氧化物质与牛血清白蛋白相互作用.pdfVIP

2007 65 Vol. 65, 2007 5 , 421429 ACTA CHIMICA SINICA No. 5, 421429 · · * ( 210038) (FS)(UV)(UF), (BSA). Lineweaver-Burk BSA (KLB)(r) BSA ; Scatchard BSA (Ka)(n); . : BSA , BSA ; BSA ; BSA ; BSA . ; ; ; Interaction between Three Antioxygenic Micromolecules and Bovine Serum Albumin WEN, Xiao-Dong LI, Ping* QIAN, Zheng-Ming YANG, Ran (Key Laboratory of Modern Chinese Medicines, Ministry of Education of PRC, China Pharmaceutical University, Nanjing 210038) Abstract The conjugation between bovine serum albumin (BSA) and three antioxygenic micromolecules of ferulic acid, calycosin and formononetin was investigated here, using fluorescence spectroscopy (FS), ul- traviolet spectroscopy (UV) and ultrafiltration (UF). The binding constants KLB and distances r of these compounds and BSA were calculated respectively, according to Lineweaver-Burk equation and energy transfer theory. And every type of conjugation effort was estimated by the calculation of thermodynamic parameters. Besides, the binding constants Ka and binding site numbers n in terms of Scatchard equation were also obtained. The results were showed as follows: the ferulic acid, calycosin and formononetin could form non-covalent compounds with BSA, which led to the static q