上海地区生猪屠宰产品中猪链球菌2型和戊型肝炎病毒两种人兽共患病病原的调查.pdf

果，在上海生猪屠宰产品中存在猪戊型肝炎病毒RNA，平均检出率为 1.4%（5/461），胆汁检出率为3.3%（2/60）、猪粪0.6%（1/160）、血 清3.2%（2/61） 、肝脏、脾脏和肠系膜淋巴结未检测到HEV RNA 病 毒。对5 个阳性样本的测序及序列系统进化分析发现，其中2 个为基 因 4 型，3 个为基因 3 型。3 株基因 3 型 HEV 和一株日本野猪分离的 基因3 型 HEV（AB222184）聚为一枝，同源性分析显示他们之间同源 性为 91.2%-98.6%，提示他们可能来源于同一祖先株。2 基因 4 型毒 株之间同源性为94.9%，与其他基因4 型毒株同源性为82.5%-87.6%， 他们单独聚为一枝在基因4 型群内构成一个亚群，本研究中发现的两 株基因4 型HEV 可能为新的亚型。 关键词： 猪链球菌 2 型，分离鉴定，毒力基因，猪戊型肝炎病毒， 基因型，同源性 INVESTIGATION OF STRETOCOCCUS SUIS TYPE 2 AND SWINE HEPATITIS E VIRUS CARRIED IN HEALTHY PIGS AND PORK PRODUCTS IN SHANGHAI SLAUGHTERHOUSES ABSTRACT Streptococcus suis type 2 （SS2 ）and swine hepatitis E virus （HEV）were the major zoonosis pathogeny carried in healthy pigs and pork products. To offer voluble reference data to food safety and health protection of related area, the carry rate of virulent Streptococcus suis type 2 and swine hepatitis E virus was investigated in Shanghai slaughterhouses. 1. The investigation of virulent Streptococcus suis type 2 from tonsillar specimens of slaughtered healthy pigs in Shanghai 475 tonsillar specimens of slaughtered healthy pigs were collected from 4 Shanghai slaughterhouses from March to November in 2006. 27 strains bacteria were identified as Streptococcus suis type 2 based on bacteria cultural characters, API20 Strep Biochemistry Identification System and PCR assays．Mainly virulent factors of the isolated strains were detected and found that only two strains were cps2/gdh+/epf-/mrp+ and others were cps2/gdh+/epf-/mrp- . The results suggested that the high virulent Streptococcus suis type 2 may be carried by slaughtered healthy pig and the carry rate was 5.7% （27/475），but no high disease-causing Streptococcus suis + + type 2 strains （MRP EF ）were isolated from 4 Sha