基于PoPD_MWCNTs_离子液体_纳米金髓过氧化物酶免疫传感器.pdfVIP

2011 年第69 卷 化 学 学 报 Vol. 69, 2011 第4 期, 438～444 ACTA CHIMICA SINICA No. 4, 438～444 ·研究论文· 基于 PoPD-MWCNTs-离子液体/纳米金的髓过氧化物酶免疫传感器 刘 蓓 逯岭松 刘成桂 谢国明* (重庆医科大学检验系 临床检验诊断学教育部重点实验室 重庆 400016) 摘要 在离子液体([EMIM]Br) 中, 将聚邻苯二胺(PoPD)-多壁碳纳米管(MWCNTs)复合物原位电聚合到金电极(Au)表 面, 利用纳米金固载髓过氧化物酶(MPO)抗体, 构建了一种用于MPO 检测的无需标记的电流型免疫传感器. 采用循环 伏安法(CV)和扫描电子显微镜(SEM)对修饰过程进行表征. 探讨了邻苯二胺单体浓度、pH 、孵育时间和孵育温度对该 免疫传感器性能的影响. 实验结果表明, 该传感器在最适条件下对MPO 响应良好, 其线性范围为0.25～350 ng/mL, 线 性相关系数为0.9985, 检出限为0.07 ng/mL. 该电流型免疫传感器具有稳定性好、灵敏度较高、特异性好、结果准确可 靠和可再生等优点, 可应用于临床检测. 关键词 髓过氧化物酶; 免疫传感器; 离子液体; 聚邻苯二胺; 多壁碳纳米管 Immunosensor for Myeloperoxidase Based on PoPD-MWCNTs-Ionic Liquid/Nanogold Modified Au Electrode Liu, Bei Lu, Lingsong Liu, Chenggui Xie, Guoming* (Key Laboratory of Ministry of Education of Clinical Laboratory Diagnostics, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016) Abstract A novel label-free amperometric immunosensor for the determination of myeloperoxidase (MPO) based on poly-o-phenylenediamine (PoPD)-multi-wall carbon nanotubes (MWCNTs)-ionic liquid/ gold nanoparticles (nanogold) modified gold electrode (Au) was constructed. Firstly, a PoPD/MWNTs composite modified Au electrode was prepared in situ electropolymerization using ionic liquid as the sup- porting electrolyte. Then gold nanoparticles were adsorbed onto the electrode surface through amino-groups on the c