抗坏血酸论文：维生素C对糖尿病大鼠肾缺血—再灌注损伤的影响.docVIP

抗坏血酸论文：维生素C对糖尿病大鼠肾缺血—再灌注损伤的影响 【中文摘要】观察维生素C(ascorbic acid, VC)对链尿佐菌素(STZ)诱导糖尿病大鼠肾缺血再灌注损伤的影响。方法：32只SD大鼠随机分成四组,其中正常血糖组(A组)8只,另外24只用STZ诱导糖尿病后随机分成3组：糖尿病组(B组),糖尿病+肾缺血-再灌注组(C组),糖尿病+肾缺血-再灌注+VC组(D组),每组8只。A组为空白对照组,B、C组每天腹腔注射0.5ml生理盐水共15天；D组每天腹腔注射VC200mg/Kg (0.5ml)共15天；15天后C、D组行缺血再灌注处理,缺血30min后再灌注24h,观察血糖、BUN、SCr、MDA、SOD、Cytc、caspase-3的变化。结果：C组血浆尿素氮、肌酐、MDA水平较D组明显升高(P0.05),SOD的含量D组较C组明显升高(P0.05)。在组织病理学方面,经VC处理可明显减少caspase-3和Cytc的表达及肾小管损伤、坏死。结论：VC能减轻STZ-诱导糖尿病大鼠肾缺血-再灌注-损伤的氧化应激、抑制细胞的凋亡及改善肾功能。 【英文摘要】Object:To investigate the protective effects of ascorbic acid on renal ischemia/ reperfusion in STZ-induced diabetic rat.Method:Thirty-two SD rats were randomly devided into four groups,groupA (n=8)and the other twenty four rats were induced diabetes by STZ, then were randomly divided into three groups with 8 rats each.Group A was control;Group B and group C rats were given normal saline 0.5ml/day,i.P, for 15 days;GroupD rats were given ascorbic acid 200mg/kg/day,i.P.,for 15 days; The left renal artery and vein of group C and group D rats were occluded for 30 min at the 18 th day, followed by 24 h of reperfusion.Results:Compared to group D,the serum BUN、SCr、MDA level significant higher in group C (P0.05).In histopathological examinations, treated with VC can significant reduce the tubular cell damage, and reduce the expresion of Cytc and caspase-3.Conclusion:Vc treatment reduces renal injury by reducing lipid oxidation and reducing cell apoptosis and improving renal function in STZ-induced diabetic rats exposed to IR. 【关键词】抗坏血酸 糖尿病 缺血-再灌注 【英文关键词】Ascorbic acid diabetes Ischemia/Reperfusion (IR) 【目录】维生素C对糖尿病大鼠肾缺血—再灌注损伤的影响 摘要 3-4 ABSTRACT 4 第1章 引言 7-9 第2章 材料与方法 9-16 2.1 实验材料 9-10 2.2 实验方法 10-12 2.3 观察及检测指标 12-15 2.4 统计学分析 15-16 第3章 结果 16-22 3.1 一般情况 16 3.2 血糖 16 3.3 肾功能 16-17 3.4 MDA 17-18 3.5 SOD 18-19 3.6 HE染色 19 3.7 免疫组化 19-22 第4章 讨论 22-24 第5章 结论 24-25 致谢 25-26 参考文献 26-28 攻读学位期间的研究成果 28-29 综述 29-34 参考文献 33-34