发展一个特定职业免疫反应.docVIP

开发类型1拟除虫菊酯杀虫剂的特效的免疫检测 Development of a class-speci?c immunoassay for the type I pyrethroid insecticides 一般的酶联免疫吸附测定法建立了ⅰ型拟除虫菊酯杀虫剂,如氯菊酯,phenothrin,resmethrin和bioresmethrin。 通过immunizing多克隆抗体生成与氯菊酯衍生物,二、2-dimethyl-3 -(5-carboxy-pent-1-en-yl)cyclopropanecarboxylic酸-(3-phenoxybenzyl)酯与thyroglobu共轭-林、牛serumalbumin或以便。 对8个不同的涂层Antiserawere筛选抗原。 antibody-antigen的结合最低的背景、氯菊酯的敏感度最高为进一步优化和测试,为的溶剂及清洁剂宽容。这I50优化免疫的30克/升为氯菊酯和20克/升为phenothrin,分别。测量不cross-reactivities对II型拟除虫菊酯,例如esfenvalerate、氟氯氰菊酯、氯氰菊酯 酯和fluvalinate氰戊菊酯。 这篇文章可用于监测研究区分类型I和II栏目中拟除虫菊酯。 引言 The synthetic pyrethroids, such as permethrin, phe-nothrin, bioresmethrin, fenvalerate and deltamethrin differ in their toxicity, photostability and chemical structure [1]. They have been widely used to control insect pests in agriculture. Among type I pyrethroids,permethrin has been widely used for the control of insect disease vectors and as a moth-proo?ng agent by the textile industry [2,3] because it is less toxic to ?sh than the type II pyrethroids. The use of pyrethroid compounds has recently increased due to the low 拟除虫菊酯的合成,如氯菊酯、苯丙氨酸-nothrin,bioresmethrin、氰戊菊酯和溴氰菊酯不同的毒性、光化学稳定性和化学物质结构[1]。他们已经被广泛地应用于控制害虫在农业生产上。在ⅰ型拟除虫菊酯,氯菊酯广泛用于控制的疾病和昆虫向量防蛀的代理通过纺织行业(2、3)因为它是毒性小的鱼比II型拟除虫菊酯。利用拟除虫菊酯类最近又增加了化合物由于低哺乳动物的毒性。因此,污染生态系统元件和食品来自农业也可能发生。因此,一个敏感的,选择性监测和快速的方法拟除虫菊酯残留物是必需的。 There are many methods for the detection of pyrethroids, such as high-performance liquid chro- matography (HPLC), gas chromatography with electron capture detector (GC–ECD) and with mass spectrometry (GC–MS). Though capable of good sensitivity, the procedure for sample preparation in such methods is very complicated, and highly skilled operations are required. Therefore, we need another effective and rapid method for analysis of many pesticides at residue levels. Hammock and Mumma reported that immunoassay could be used for pesticide residue analysis and screening. Due to the lower cost and higher sample throughput achievable compared to instrumental analyses, ELISA can be an