201305061 论著胸部撞击伤对声门紧闭和开放状态大鼠肺微血管VE-cadherin 表达影响.docVIP

201305061 论著闫进463000 河南 驻马店中国人民解放军第159中心医院心胸外科-钙粘附蛋白（VE-cadherin）表达的影响，了解撞击伤后肺微血管通透性的改变。方法 利用荧光素钠法检测肺组织的微血管通透性，动态观察撞击伤对细胞间连接的特有成分VE-cadherin表达的影响。结果 撞击侧（右肺）肺微血管通透性均较伤前显著增高，各组在伤后同一时相点的值与正常对照组相比差异有统计学意义（P＜0.01），声门紧闭组荧光素钠的含量高于声门开放组，差异有统计学意义（P＜0.01）。胸部撞击伤后大鼠肺微血管内皮细胞VE-cadherin的表达降低，与对照组相比差异有统计学意义（P＜0.01）。结论 声门紧闭状态下撞击伤后肺微血管通透性高于声门开放状态，VE-cadherin在撞击伤后肺微血管内皮细胞中的表达下调，使得内皮细胞之间的连接疏松，通透性增加。 [关键词]胸部撞击伤；肺微血管；VE-cadherin [中图法分类号] [文献标志码] A Expression of VE-cadherin in microvascular endothelial cells in the Wistar rats lung injuries induced by blunt chest trauma under closure of glottis Wu Qiuping1，Department of Thoracic Surgury， Xinqiao Hospital of the Third Military University， Chongqing 400037Department of Cardiothoracic Surgury，Peoples Liberation Army, Henan 463000） [Abstract] Objective To comprehend the alteration of the pulmonary microvascular permeability after Wistar rats lung impact injury by detecting the expression of endothelial VE-cadherin in pulmonary microvascular endothelial cells. Methods The alteration of the microvascular permeability after BCT was examined by means of fluorescein sodium （FINa） method and by detecting the expression of endothelial VE-cadherin in pulmonary microvascular endothelial cells. Results The results revealed that at any one time point the content of FINa was higher in the group of glottis closure than that in the open one（P＜0.01）. The expression of Wistar rats endothelial VE-cadherin in pulmonary microvascular endothelial cells is decreased after chest impact injury, compared with the control group had significant difference (P 0.01). Conclusions When the glottis was closed the microvascular permeability and edema of lung tissue were more severe than that when the glottis was open .The expression level of VE-cadherin was down regulated in endothelial cells resulting in the loosed conjunctions between endothelial cells and subsequent increase of permeability. [Keywords]Thoracic trauma；pulmonary microvas