HPLC同时测定苦豆子中槐定碱、氧化槐果碱和氧化苦参碱的含量.pdfVIP

维普资讯 第32卷第24期 中 国 中 药 杂 志 V01．32．Issue 24 2007年 l2月 ChinaJournalofChineseMateriaMedica December，2007 Studieson solidphaseextractionmethodofaristolochicacidsand aristololactamsin raturine ZHAODian．hong，QULei，WANGXuan，LIXiao．mei，XUJun-yu，CAIShao—qing (1．SchoolofPharmaceuticalSciences，PekingUniversity，Befiing100083，China； 2．RenalDivision，FirstHospital，DepartmentofMedicines，PekingUniversity，Beifing100034，hCina) [Abstract] Objective：TodevelopaurinepretreatmentmethodofSolidPhaseExtraction(SPE)forthequantitativedetermina- tionofanumberofaristolochicacids(AAs)andaristololactams(ALs)inraturine．Method：TheHPLCpeak~uFeaofAA—I，从 - Ⅱ，AL．IandAL．II，andothersixteenAAsandALswaschosenasevaluatingindextostudytl1eextractresultsoffiveSolidPhase Extractioncolumns(AgilentC18／100mg，AlhechHC18／100mg，AlhechC18／100mg，AlhechC18／300mgnadAgilentPhenyl／200 mg)comparatively．Theinfluencesoftwowashingsolvents(waternad1％ aceticacid-0．02％trieth)rlaminesolution)nadsevenelu— tingsolvents(ehter，acetone，chloroform，ethylacetate，dichloromethnae，mehtanolnadacetonitrile)onextractresultsofAAsand AI鼻areocmparativelystudiedwitl1theextractingrecoveriesofAA—I，AA一Ⅱ，AL·InadAL-I1asindicators．TheHPLCpeakraea 0fAA—I，AA一Ⅱ，AL—InadAL—II，andothersevenAAsandALswiht goodsepraationbeingtragets，several factorswhichaffect extractingefficiencyofnaalytes，includingactivatingvolume，cleansingvolume，washingvolumena delutingvolume，raeoptimizedby orthogonal designexperimentswiht fourfactors atthreelevels．Result：Th eestablishedmehtodofSPE isasfollows：AgilentPhenyl SPE columnof200mg，activatingwith1．0mLmethanol，clenasingwim 1mLwater，adding1．0mLraturinesample，washingwitl1 0．8mLl％ aceticacid0．02％ triethylamin