翡翠贻贝内脏团抗氧化防御系统对菲胁迫生物响应.docVIP

翡翠贻贝抗氧化防御系统对菲胁迫生物响应(2.0、10.0、50.0 μg L-1）Perna viridis）Biological Effects of Phenanthrene on the Antioxidant Defense System of Viscera in Green-lipped Mussel(Perna viridis) YANG Tao1,2, CHEN Haigang1, CAI Wengui1, QIN Jiefang1,2, JIA Xiaoping1** (1South China Sea Fisheries Research Institute, CAFS; Key Laboratory of Fishery Ecology Environment, Guangdong Province of China；Key Field Scientific Experimental Station of South China Fishery Resource and Environment, Ministry of Agriculture, Guangzhou 510300, China） (2 Marine Science college of Shanghai Ocean University, Shanghai 201306, China) Abstract Changes on activities of antioxidantive enzymes (SuPeroxide dismutase (SOD),Glutathione Peroxidase (GSH-Px),Catalase (CAT)) and content of malondialdehyde(MDA)in viscera of green-lipped mussel （Perna viridis）were studied after exposured with different phenanthrene concentrations of 2.0、10.0、50.0 μg L-1However, the contents of MDA increased during the whole exposured time, and reached the peak level after 15d expusured, and it will be more significant when its concentration is higher for this three indicators. As a result, SOD、CAT、GSH-Px activity and MDA content are suit for monitoring the oxidative damage of aquatic life by phenanthrene exposure. The four indicators recover to the control level after transferred to the clean seawater for 7 days, which illustrate that green-lipped mussel can make a good self-repair to phenanthrene stress. Fig 4, Tab1, Ref 19 Keywords phenanthrene; green-lipped mussel(Perna viridis); viscera; superoxide dismutase(SOD); glutathione peroxidase (GSH-Px); catalase (CAT); malondialdehyde(MDA) 近些年来，由于石油泄露、城镇污水排放以及煤燃料的不完全燃烧，水体环境中的PAHs(多环芳烃类化合物)污染日趋严重，而菲这种典型的PAHs化合物，在水体中的分布也已相当广泛，在我国，辽河流域中检测到的菲含量为4.44 ~25.04 ng g-1，长江流域为16.78~64.11 ng g-1，而珠江三角洲地区河流表层菲含量则高达1460.61ng g-1 [1,2]。菲对水生生物具有很强的毒性，而且具有环境激素的性质。Correia发现，在高浓度的菲曝露条件下，鲫鱼表现出昏睡，行为迟钝的现象[3]。Hannam等学者通过研究发现，菲能通过降低细胞膜的稳定性和吞噬能力，继而对大海扇蛤的免疫能力造成不利影响[4]；徐文菊等通过菲对鳗鲡进行14天的水体曝毒后，观察到鳗鲡肝脏细胞肿大，细胞核边缘化或者消失，脾脏血红细