基于水溶性共轭聚合物蛋白质检测.pdfVIP

21 11 化 学 进 展 Vol.21No. 11 2009 11 PROGRESS IN CHEMISTRY Nov., 2009 基于水溶性共轭聚合物的蛋白质检测* 贺 芳 王 树* * ( 中国科学院化学研究所 有机固体重点实验室 北京100190) 近年来, 共轭聚合 作为生 传感元件, 在生 大分子( 如核酸蛋白质) 特异性识别和检测方 面的研究越来越受到人们的关注共轭聚合 具有强的光捕获能力, 具有倍增光学响应性, 可用来放大荧光 传感信号,大大提高检测的灵敏度, 为生 传感器的发展提供了新的传感模式基于共轭聚合 的新型生 传感器在医疗诊断环境检测以及国家安全防御等方面具有广泛的应用前景本文简要介绍了共轭聚合 的荧光信号放大机制以及在蛋白质酶抗原-抗体检测方面的应用最后对共轭聚合 在蛋白质检测方面 的未来发展趋势进行了展望 共轭聚合 荧光信号放大 蛋白质检测 : O644; O63; O629.7 : A : 1005-281X(2009) 11-2372-07 Water- Soluble Conj ugated Polymers for Protein Detections * * He Fang Wang Shu (Key Laboratory of Organic Solids, Institute of Che istry, ChineseAcade y of Sciences, Beijing 100190, China) Abstract Conjugated poly ers (CPs) are characterized by their delocalized electronic structures. The excitation energy along the whole backbone of CPs transferring to the reporter results in the a plified fluorescence signal, which akes the tobe used as theoptical platfor s in highly sensitiveche ical and biologicalsensors. This new technology is receiving ore and ore research and application interest due to the rapidly increasing de ands of clinical diagnosis, environ ental analysis and national defense. In this review, the echanis of signal a plification of CPs and their applications in sensitive protein detection are introduced. Further ore, the future research directions for protein detections based on CPs are also presented. Key words conjugated poly ers; signal a plification; protein detection a plification of CPs Contents 4 Conclusion and prospects 1 Introduction