海洋来源细菌CB101抗肿瘤活性次级代谢产物初步的研究.docVIP

海洋来源细菌CB101抗肿瘤活性次级代谢产物的初步研究 蔡生新1，，，顾谦群* （教育部海洋药物重点实验室，中国海洋大学医药学院，青岛，266003) 摘要：目的 方法硅胶柱色谱及HPLC等分离手段理化性质及波谱学手段进行化学结构鉴定，以SRB法评价化合物的抗肿瘤活性 hexan-2-one (3)，吲哚 (4)，trisindoline (5)，indole-3-ethanol (6)，2,2-di(3-indolyl)-3-indolone (7)，2-[2,2-bis(1H-indol-3-yl)-ethyl]phenylamine (8)，1,1,1-tris(3-indolyl)methane (9), 其中化合物1-3为新化合物且具有较弱的抗肿瘤活性。 关键词：来源真菌；次级代谢产物；抗肿瘤活性 , LI De-hai1, ZHU Tian-jiao1, WANG Feng-ping2,XIAO Xiang2, GU Qian-qun1* (1. Key Laboratory of Marine Drugs, Chinese Ministry of Education; Institute of Marine Drugs and Food, Ocean University of China, Qingdao 266003, P.R. China;2. Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, The State Ocean Administration, Xiamen, P.R. China) ABSTRACT: OBJECTIVE Investigation of the secondary metabolites of marine derived bacterium CB101(Aeromonas sp.) and evaluate their antitumor activity. METHODS Bioactive fractions were obtained from the crude extract of CB101(Aeromonas sp.) through a bioassay-guided fractionation and the chemical constituents of the fractions were investigated and purified by the combined use of column chromatography with silica gel and Sephadex LH-20, the preparative HPLC. Their structures were established by spectroscopic methods. Their antitumor activities were evaluated by SRB and MTT methods. RESULTS AND CONCLUSION Nine indole-derived compounds (1-9) were isolated and identified as 4-[bis-(3-indolyl)methyl]quinoline (1)，2,2-bis(3-indolyl)ethyl-butyrate (2), 4-(3-indolyl) hexan-2-one (3)，indole (4)，trisindoline (5)，indole-3-ethanol (6)，2,2-di(3-indolyl)-3-indolone (7)，2-[2,2-bis(1H-indol-3-yl)-ethyl]phenylamine (8)，1,1,1-tris(3-indolyl)methane (9), respectively. Among these compounds, 1-3 are new compounds and they have weak antitumor activity. Key words: marine derived bacterium; secondary metabolites; indole-derived compounds; antitumor activity 海洋微生物多生活在高盐、高压、低营养、低温(特别是深海)和无光照等特殊环境之中,为适应这些生命的极限环境, 海洋微生物已发展出独特的代谢方式,也使其产生的海洋天然产物具有种类繁多、结构特异及活性强等特点,这为寻找海洋生物活性物质提供了丰富