芬太尼预处理对家兔急性失血性休克心肌损伤的影响.docVIP

芬太尼预处对损伤响刘晓 姚立农 伟 观对损伤响 16只家兔随为盐预处组C组，8预处组F组，8Wigger’s改良法制作家兔失血性休克模型。放血前15min两组别给盐维状态90 min（T2）和90min（T3） 时动脉血用学测钙蛋I (cTnI)的浓结cTnI的浓P0.01），组间较C组的cTn-I升高幅度明显高于F组P0.01）；病理学光镜检查F组心肌损伤较C组减轻 结论预处对损伤 【关键词钙蛋I；心肌损伤Effect of fentanyl on myocardial injury in rabbits with acute hemorrhagic shock [Abstract] AIM ：To investigate the effect of fentanyl on ischemia-hypoxia injury in rabbit heart with acute hemorrhagic shock.METHODS： Sixteen healthy white rabbits were randomly divided into two groups with 8 each:control group(Cgroup)and fentanyl preconditioning group(F group).Acute hemorrhagic animal model was established according to the Wigger’s method.15min before bloodletting，the same volume of saline and fentanyl were given to group C and group F respectively. Keep the state of hemorrhagic shock for 90 min and take arterial blood before bloodletting（T0）,60min after bloodletting (T2) and 90min after bloodletting(T3) for determination of serum cardiac Troponin I( cTnI) with chemiluminescence method. Morphological change of the heart was observed by light microscopy. RESULTS： The serum concentrations of cTnI increased significantly in two groups at T2 and T3 compared with T1( P 0.01) . cTnI values at T2 and T3 in group F were lower those in group C(P0.01)。myocardial injury in group F was less severe than that in group C.CONCLUSION：Fentanyl preconditioning can reduce myocardial injury with acute hemorrhagic shock 【Key words】 fentanyl ;preconditioning;hemorrhagic shock; cardiac Troponin I; myocardial injury 0引言 失血性休克时会许脏损伤脏损伤预关，在临参这类抢临点护脏发证实等许药拟血预处对损伤护对单纯损伤护旨在观对时损伤响 1 材料与方法 1．1材料 健康家兔16只，雌雄不拘，体重2.0-2.5Kg，由第四军医学动实验 生产。动组 16随为两组对组8C组预组8F组两组15 min分别给盐25μg/Kg）。耳缘静脉给钠30mg/Kg开侧动脉备动Wigger’s法制作而成。在10min内动脉血压平40mmHg，放血量C组为39.00±6.28），F组为40.24±4.88），两组比较无显著性意义(P0.05),过放输血维为40mmHg min，并观90min。 1．2．2 观给药前60 min 和90min, 采集动脉血用）检测钙蛋I（cTn-I）的水平。（3）心肌组织结:实验结时头1mm 3 心肌组织,规备电镜标,在Hu - 12A 透射电镜(HITACHI ,产) 观 统计学处 表示, 组内及组间比较采用 t 检验, P 0.05 表示差异有显著性。 2 结两组动项备模时无