脂肪酶连续填充式反应优化.pdfVIP

Hindawi Publishing Corporation Journal of Biomedicine and Biotechnology Volume 2011, Article ID 950725, 6 pages doi:10.1155/2011/950725 Research Article Continuous Production of Lipase-Catalyzed Biodiesel in a Packed-Bed Reactor: Optimization and Enzyme Reuse Study Hsiao-Ching Chen,1 Hen-Yi Ju,1 Tsung-Ta Wu,1 Yung-Chuan Liu,2 Chih-Chen Lee,2, 3 Cheng Chang,4 Yi-Lin Chung,4 and Chwen-Jen Shieh4 1 Department and Graduate Program of Bioindustry Technology, Dayeh University, Chang-Hua 515, Taiwan 2 Department of Chemical Engineering, National Chung Hsing University, Taichung 402, Taiwan 3 Derlin Biotech Corporation Ltd., Yun-Lin 640, Taiwan 4 Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan Correspondence should be addressed to Chwen-Jen Shieh, cjshieh@nchu.edu.tw Received 29 June 2010; Accepted 2 September 2010 Academic Editor: Nico Boon Copyright © 2011 Hsiao-Ching Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. An optimal continuous production of biodiesel by methanolysis of soybean oil in a packed-bed reactor was developed using immobilized lipase (Novozym 435) as a catalyst in a tert-butanol solvent system. Response surface methodology (RSM) and Box- Behnken design were employed to evaluate the effects of reaction temperature, flow rate, and substrate molar ratio on the molar conversion of biodiesel. The results showed that flow rate and temperature have significant effects on the percentage of molar conversion. On the basis of ridge max analysis, the optimum conditions were as follows: flow rate 0.1 mL/min, temperature 52.1◦C, and