抗流感病毒小RNAs研究进展.pdfVIP

HEREDITAS (Beijing) 2012 5 , 34(5): 526―532 ISSN 0253-9772 综 述 DOI: 10.3724/SP.J.1005.2012.00526 抗流感病毒小RNAs 研究进展 郑维豪, 林志强, 卓敏, 杜红丽, 王小宁 , 510006 流行性感冒是一类由流感病毒引起的呼吸道传染病, 通过季节性流行或全球性爆发严重威胁着人类健 康。目前防治流感的主要方法是疫苗和药物, 但存在神经毒性、肠胃副作用、易耐药等诸多限制因素。新的技 术特别是小RNAs 介导的RNA 干扰(RNAi)技术, 因其具有高效、特异、快速等特点, 已成为抗病毒治疗的候选 方法之一。随着近年来流感病毒的流行, 应用小 RNAs 抗流感病毒的报导越来越多, 其中靶向 PA 、NP 和 M2 的PA-2087, NP-1496 和M-950 是目前报道的抑制流感病毒效果最好的 siRNA 。靶向不同流感病毒基因保守区 域的siRNA 具有更广泛的病毒毒株抑制效果, 靶向不同基因的siRNAs 联合使用可取得更好的病毒抑制效果。 文章就目前siRNAs 和miRNAs 在抗流感病毒方面的研究进展及RNAi 治疗的前景和问题进行了综述。 流感病毒; 小RNAs; siRNAs; miRNAs; RNAi Research progress on influenza antiviral small RNAs ZHENG Wei-Hao, LIN Zhi-Qiang, ZHUO Min, DU Hong-Li, WANG Xiao-Ning School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China Abstract: Worldwide influenza caused by influenza virus is a respiratory disease which threats the public health by seasonal epidemics or global influenza outbreak. Vaccines and drugs are current therapies, but there are many restricted factors such as neurotoxicity, side effects of gastrointestinal, and drug resistance. New technologies, particularly RNAi me- diated by small RNAs, has become a potential and robust method in influenza antiviral research for its high efficiency, spe- cific, and speedy. Following the spread and epidemic of the influenza virus, application of small RNAs into influenza anti- viral research has been reported increasingly. The small RNAs, PA-2087, NP-1496, and M-950, which targets PA, NP, and M2 genes, respectively, are the most effective anti-influenza siRNAs up to now. siRNA of targeting conservative region of