不同干露时间对三疣梭子蟹非特异性免疫系统的影响【毕业论文】.doc

本科毕业论文 （20 届） 不同干露时间对三疣梭子蟹非特异性免疫系统的影响 专业：水产养殖学 目录 中文摘要…………………………………………………………………………3 英文摘要…………………………………………………………………………4 1 前言………………………………………………………………………… 6 2 材料和方法………………………………………………………………… 6 2.1 材料………………………………………………………………………7 2.2 方法………………………………………………………………………7 2.2.1 干露实验及采样………………………………………………………7 2.2.2 血淋巴细胞观察和计数………………………………………………7 2.2.3 血清中酶活力和蛋白测定……………………………………………8 2.2.4 干露实验中梭子蟹成活率数据处理不同血淋巴的干露不同时间对暂养梭子蟹免疫酶活力和血清蛋白影响干露对梭子蟹干露实验梭子蟹最终成活率不同干露时间对三疣梭子蟹非特异性免疫的影响 本实验将三疣梭子蟹离水干露后，进行，对三疣梭子蟹血细胞总数、血清蛋白、酸、碱性磷酸酶、超氧化物歧化酶等非特异性免疫系统相关指标正常梭子蟹的血淋巴凝固时间平均血淋巴凝固时间为38s，干露梭子蟹血淋巴凝固时间随干露时间的延长发生明显变化干露血细胞总数P<0.01），而大颗粒细胞比例相反则有显著上升(P＞0.05）2h干露组1h干露组差异极显著（ <0.01）；1h干露血细胞总数较恢复前数量有所上升对照组差异显著（ <0.05）2h干露血细胞总数恢复前无，差异均极显著（ <0.01）。大颗粒细胞比例干露组和对照组之间变化明显，干露组低于对照组（ <0.01）；大颗粒细胞占血细胞总数的百分比差异显著，干露组对照组（ <0.05）；蛋白含量干露1h蛋白含量变化不大，而干露2h后蛋白含量差异显著（P<0.05）；酸性磷酸酶活性随干露时间延长略有增强，但总的来看与对照组差异不显著（P＞0.05）；超氧化物歧化酶活力变化，Study on influences of air-exposure on the non-specific immunological parameters of Portunus?trituberculatus [Abstract] This experimental study on influences of air-exposure on the non-specific immunological parameters of Portunus?trituberculatus,Make the Portunus?trituberculatus air-exposure 1h, 2h, Mark group A and group B,portunus total hemocyte count、serum protein, acid, alkaline phosphatase, superoxide dismutase enzymes non-specific immune system-related indicators for tracking and detection. The results showed that: the hemolymph coagulation time of normal crab is an average of 38s, the hemolymph clotting time of concha crab is change significantly by the time of concha; total hemocyte count in concha experimental group and control group was significantly lower (P <0.01), and large granulosa cells in contrast ratio were significantly increased (P> 0.05); In the group B, total hemocyte count than the group A, Significant difference (p <0.01); After restored: Although total hemocyte count in group A has an increase in the number, But still low compared with the control group, Significant difference