HPLC同时测定丹参及其制剂中4种酚酸类成分的含量.pdfVIP

HPLC 同时测定丹参及其制剂 中4 种酚酸类成分的含量 侯媛媛 ，黄晓睛，兄若冰 ’( 四川大学华西药学院，成都610041) 摘要: 目的 建立同时测定丹参药材及其制剂 中丹参素、原儿茶醛 方法 色谱柱:Inertsil C8- 3 柱;流动相: 乙睛一水一甲酸(24:76:0.4) ;流速: 1.0 mL min - ;检刚波长:285 nm。结果 在此色谱 条件下4 种酚酸类成分可完全分离。丹参素、原儿茶醛、丹酚酸A, 丹酚酸 B 的线性范围分别为:8 - 400 ( r = 0. 999 8) , 4 - 200 ( r = 0. 999 4 ) ，4 - 2 0 0 1 = 1.000 0) 和4 一200 m ·L一(‘r = 1. 000 0) 0 4 种成分在药材和制剂 中的回收率均在98. 5% - 10 1. 0% , RSD 2 . 0 % 。结论 该方法简便、准确、快速 ，可同时测定丹参药材及其制剂中4 种酚酸类成分的含量。 关键词: 高效液相色谱法; 丹参;丹参素;原儿茶醛;丹酚酸A; 丹酚酸B;含量测定 中图分类号:R9 17 文献标识码 :A 文章编号: 100 1- 2494 (2007 ) 14 一1108 - 04 Simult aneous Det er min ation of Four Phenoil c Acids in Salvia miltiorrh iza B e. and Its Pr epar ations by H P L C HOU Yuan-yuan ，HUANG Mao一in ，CHAO Ruo-bin (‘West China School of Pharmacy, Sichuan University, Chen du 6 1004 1，China ) ABSTRACT :OBJECTIVE To develop a HPLC method for the simultaneous determination of danshensu , protocatechuic aldehyde, salvianolic acid A, salvianolic acid B in Salvia miltiorrhiza B e. and its preparations. METHODS The analysis was carried on a col- umn of Inertsil C:一with a mobile phase of acetonitrile-water-formic acid(24:76:0.4) at the lf ow rate of 1.0 mL ·min .‘The detec- tion wavelen thwas285run. RESULTS Thelinearitywasobtainedover theran eof8一400m ·L一(r‘二0.999 8) for danshensu, 4 一200 m ·L一’(r 二0.999 4) for protocatechuic aldehyde , 4 一200 m ·L一’(r = 1.000 0) for salvianolic acid A and 4 一200 m · L一’(r = 1. 000 0) for salvianolic acid B, respectively. The avera e recoveries of four phenolic acids in Salvia miltiorrhiza B e. And its preparations determined were all in 98. 5% 一101. 0%a ，and the RSDs were less than 2.0% . CONCLUSION The method is simple , accurate and rapid. It may be suitable for the usual quality control method of Salvia miltiorrhiza B e. and its preparations. KEY WORDS:HPLC;Salvia miltiorrhiza B e. ;danshensu ;protocatechuic aldehyde ;salvianolic acid A;salvianolic acid B;quantitative analysis