Establishment of classical conditioned papillary dilations in conscious and fixed cats and study of .docVIP

201304088 论著 清醒制动家猫条件化瞳孔扩大反射的建立及其习得规律研究 张浪千，蒋 政，胡 博，隋建峰（400038 重庆，第三军医大学基础医学部基础医学教学实验中心） [摘要]目的 建立家猫条件化瞳孔扩大反射模型并研究其习得规律。方法 以声音作为条件刺激（CS），尾部电击作为非条件刺激（US），对3只健康成年家猫分别用CS-US间隔0 ms和900 ms两种模式训练。用红外瞳孔测量装置实时监测瞳孔面积变化并记录。结果 在900msCS-US刺激间隔训练中家猫习得了条件化瞳孔扩大反射，在经过10次、30次配对训练后其习得率分别为26.67%、53.33%， 50次配对训练后习得率达76.67%。在随后的30次消退训练中，习得率呈下降趋势。结论 家猫条件化瞳孔扩大反射的习得与CS-US刺激间隔和CS刺激数量有关。这种反射能在较少配对训练后就习得，但消退也较快。 [关键词]条件化瞳孔扩大反射；家猫；刺激间隔；习得率 [中图法分类号]R338.6；R338.61R33-33 Establishment of classical conditioned papillary dilations in conscious and fixed cats and study of related acquisition rule Zhang Langqian , Jiang Zheng , Hu Bo , Sui Jianfeng（Experimental Center of Basic Medicine , Third Military Medical University , Chongqing , 400038 , China） [Abstract] Objective To establish a model of classical conditioned pupillary dilations in conscious and fixed cats and to analyze the related acquisition rule. Methods With sound as the conditioned stimulus（CS）, tail shocking as the unconditioned stimulus , trained the three health adult cats with CS - US interval 0 ms and 900 ms models. With infrared pupil measurement device and record the pupil area changes real-time.Results The cat acquired classical conditioned pupillary dilations in the 900ms CS - US stimulus interval model, After 10 times, 30 times pairing trainings ,the acquisition rate is 26.67%, 53.33% ,after 50 times trainings, the acquisition rate is 76.67%. In the following 30 times fading trainings, acquisition rate trended down.Conclusion The acquisition of cats related to CS-US interval and CS stimulus amount in the classical conditioned pupillary dilations. This kind of reflection can be acquired in few pairing training s, but faded quickly. [Key words] classical conditioned pupillary dilations ;cats; CS - US stimulus interval; acquisition ra te [通讯作者隋建峰，电话（023E-mailjfsui2003@  瞳孔反射是一类重要的内脏反射，包括瞳孔扩大和缩小反射两种方式，根据反射的获得特点它们又可分为非条件性和条件性反射两类。非条件性瞳孔反射例如瞳孔对光反射，已被广泛应用于神经核精神等疾病的临床检查和诊断。条件化瞳孔反射则被少量应用于动物实验研究。早期， Gerall曾做过正常猫和中筒