双酚A对SD大鼠卵巢毒性作用实验的研究.pdfVIP

606 2011 4 18 4 Practical Preventive Medicin e, Apr. 2011, V l 18, N . 4 : 1006- 3110( 2011) 04- 0606- 03 论 著 双酚A 对SD 大鼠卵巢毒性作用实验研究 1 1 1 2 谭艳芳 , 陈 锋 , 曾 鸣, 周支香 : A SD 48 4 SD , BPA , , 180 d( E ) ( P) ( T) 2 3 , HE ; ER P53 , ( E ) ( P) , ( T) 2 , , ; : ER P53 SD , - BPA SD , SD ( E ) ( P) ( T) , ER P53 , SD 2 : 双酚A ; 卵巢; SD 大鼠 : R- 332 : A StudyonToxicityof Bisphenol A to Ovaries of Female Sprague- Dawley Rats TAN Ya n - f ang , CHEN Feng , ZEN G Ming , et a l . ( School of P ublic Health, University of South China , Hengy a ng 42 1001, Hunan , China ) Abstract: Objective To preliminarily explore the toxicity of different doses of bisphenol A ( BPA) to ovaries of female Sprague- Dawley rats. ethods Forty- eight four- week- old female Sprague- Dawley rats were randomly divided in to 4 groups based on body weight. The 4 treatment groups were gavaged with0, 100, 200 and 400 mgkg BPA, respectively, every other day for 6 months. Serum estradiol ( E2) , progesterone ( P) , and testosterone ( T) were detected by en ymelinked immunosorbent assay. The histostructures of rat ovaries were observed under light- microscope by HE staining. The expres sion of estrogen receptor ( ER) and P53 in the ovaries was examined by immunohistochemical assay. Results As com pared with control group, the serum levels of E2 and P inrats treated with different doses of BPA were increased, while T lev el was decreased with the increasing doses of BPA and there was a negative correlation betwe