二维相关荧光光谱技术.pdfVIP

第 18 卷 第 12 期 化　学　进　展 Vol. 18 No. 12 2006 年 12 月 PROGRESS IN CHEMISTRY 　Dec. , 2006 二维相关荧光光谱技术 余　婧　武培怡 (复旦大学高分子科学系 聚合物分子工程教育部重点实验室 　上海 200433) 摘 　要 　从发展历史、计算方程、一般规则和特有性质等方面系统地介绍了近年来在二维相关荧光光谱 技术方面的方法探索和应用进展。以不同的外扰方式 ,如浓度、激发波长、猝灭以及其他外扰方式如 pH 等 分类 ,举例阐述了二维荧光相关光谱的可操作性及其应用 ,并与普通一维荧光光谱比较 ,说明了二维荧光相 关光谱技术的优势。 关键词 　二维相关荧光光谱技术 　相关分析 　外扰 ( ) 中图分类号: O657. 3 　文献标识码 : A 　文章编号 : 1005281X 2006 TwoDimensional Fluorescence Correlation Spectroscopy Yu Jing 　Wu Peiyi ( The Key Laboratory of Molecular Engineering of Polymers , Department of Macromolecular Science , Fudan University , Shanghai 200433 , China) Abstract 　The technique and progress in twodimensional fluorescence correlation spectroscopy is systematically introduced in the aspects of history , mathematic equations , general regulation and characteristic properties. The introduction is sorted in the range of different perturbations , such as concentration , excitation wavelength , fluorescence quenching and the pH. There are several examples in each case helping to illustrate the operability and application of the new technique. Additionally , the new technique is also compared with the traditional onedimensional fluorescence spectroscopy , which indicates the advantage of the twodimensional fluorescence correlation spectroscopy clearly. Key words 　twodimensional fluorescence correlation spectroscopy ; correlation analysis ; perturbation Noda [3 ] 于 1986 年就二维 NMR 技术理论