4 种红景天植物的组织培养研究[J]刘春朝.docVIP

文库下载 免费文档下载  HYPERLINK / / 　　 本文档下载自 HYPERLINK / 文库下载网，内容可能不完整，您可以点击以下网址继续阅读或下载：  HYPERLINK /doc/68d34688a0116c175f0e480a.html /doc/68d34688a0116c175f0e480a.html 4种红景天植物的组织培养研究 西北植物学报,2006,26(10):2023-2027　　文章编号:100024025(2006)1022023205 4种红景天植物的组织培养研究 刘海军1,2,郭　斌1,3,晏　琼1,刘玉军2,刘春朝1,33 (1中国科学院过程工程研究所生化工程国家重点实验室,北京100080;2北京林业大学生物科学与技术学院,北京100083;3 中国科学院研究生院,北京100039) 摘　要:以大花红景天、云南红景天、长鞭红景天和库页红景天的茎和叶为外植体进行组织培养,结果表明:大花红景天以茎为外植体诱导芽效果最好,其它3种红景天以叶为外植体诱导芽效果最好.云南红景天和长鞭红景天适合的芽诱导激素组合是0.1mg/LNAA和2.5mg/L62BA的组合,在该激素水平下两种红景天的出芽频率分别达到71%和84%;大花红景天和库叶红景天适合的芽诱导激素组合是0.5mg/LNAA和2.5mg/L62BA的组合,在该激素水平下两种红景天的出芽频率均达到80%.长鞭红景天和库叶红景天在添加IBA成完整植株,生根率分别达到87%和73%;经过炼苗后,66%.关键词:红景天;组织培养;再生植株中图分类号:Q813.1 2;Q949.751.1 文献标识码:A RhodiolaSpecies Ljun1,GUOBin1,3,YANQiong1,LIUYu2jun2,LIUChun2zhao1,33 (1NationalKeyofBiochemicalEngineering,InstituteofProcessEngineering,ChineseAcademyofSciences,Beijing100080,China;2CollegeofBiologicalSciencesandBiotechnology,BeijingForestryUniversity,Beijing100083,China;3GraduateSchooloftheChineseAcademyofSciences,Beijing100093,China) Abstract:ThestemsandleavesofRhodiolacrenula/doc/68d34688a0116c175f0e480a.htmlta,Rhodiolayunnanensis,RhodiolafastigiataandRhodiolasachalinensiswereinvitroculturedastheexplants,thestemsofRhodiolacrenulataandtheleavesofRhodiolayunnanensis,RhodiolafastigiataandRhodiolasachalinensiswerefoundtobethebestpartsforshootinduction.TheoptimalhormonecombinationforshootinductionofRhodiolayunnanensisandRhodiolacrenulatawas0.1mg/LNAAplus2.5mg/L62BAandtheshootinductionpercentagesofthetwospecieswere71%and84%withtheoptimalhormonecombination,respectively;Theoptimalhor2monecombinationforshootinductionofRhodiolafastigiataandRhodiolasachalinensiswas0.5mg/LNAAplus2.5mg/L62BAandtheshootinductionpercentagesofthetwospeciesreached80%withtheoptimalhormonecombination;theinducedshootsofRhodiolafastigiataandRhodiolasachalinensiswereeasytorootonthemediumwithIBAaddedandtherootingpercentageswere87%and73%,respectively;AfterhavingbeenhardenedtheregenerationplantletsofRho