蜡状芽孢杆菌HY-1降解甲基对硫磷和毒死蜱的影响因素研究.pdf

农业环境科学学报2010，29(3)：437—443 Journalof Science Agro—Environment 蜡状芽孢杆菌HY一1降解甲基对硫磷和 毒死蜱的影响因素研究 段海明，王开运，王 冕，姜莉莉，乔康，任学祥 (山东农业大学植物保护学院，农药毒理与应用技术省级重点实验室，山东泰安271018) 摘要：采用富集驯化培养和紫外分光光度计定量的方法，从农药生产企业的废水处理系统中分离筛选出1株能够降解甲基对硫 磷和毒死蜱的蜡状芽孢杆菌(Bacillus 基对硫磷初始浓度为10～50 菌株对甲基对硫磷的降解。HY一1降解毒死蜱的适宜条件为：葡萄糖浓度6 g·L，培养温度30～35oC，pH为7．0，毒死蜱初始浓度 80～200 毒死蜱的适宜条件相类似，只是降解所需的最适葡萄糖浓度和底物浓度不同。 关键词：蜡状芽孢杆菌；有机磷农药；生物降解；影响因素 中图分类号：X172文献标志码：A 文章编号：1672—2043(2010)03—0437—07 ofBacilluscereusHY一1to and Characteristics Degradative Methyl-parathionChlorpyrifos DUAN Hai—ming，WANGKai—yun，WANGMian，JIANGLi—li，QIAOKang，RENXue—xiang 7 ofPlant ofPesticide and an (College Protection，KeyLaboratory ToxicologyApplicationTechnique，ShandongAgriculturalUniversity，Tai 271018，China) bacterial cereus isolatedfromthetreatmentofthe andits Abstract：Astrain，BacillusHY-1，was system pesticideenterprisesdegradative characteristicsto and werestudied themethodsofenrichmentandtheultraviolet methyl-parathionehlorpyrifos byusing culturing spec- determination．neresultsshowedthatthebacteriacouldutilizethe or asthe trophotometer methyl-parathionehlorpyrifosuniquephosphorus source．Theconditionsfor wereas of initialof ini- follows：the 30-35℃，the 6-8，the optimal degradingmethyl-parathion temperature pH concentration andtheinoculationamountof20％inthe results tialmethyl一parathionofl0-50mg·L_1 experiment(v／v)．The the conditionsfor wa